癌组织血管生成素1和Smad同源物9基因表达与肺腺癌患者肿瘤转移侵袭行为及预后的关系

The relationship between angiopoietin 1 and Smadhomolog 9 gene expression in cancer tissues and tumor metastasis, invasion behavior and prognosis in patients with lung adenocarcinoma

目的:探讨肺腺癌患者癌组织血管生成素1(ANGPT1)、Smad同源物9(Smad9)基因表达与肿瘤转移侵袭行为及预后的关系。方法:选取山东第一医科大学附属成武医院2018年10月至2019年12月诊治的60例肺腺癌患者作为研究对象,比较癌组织与癌旁组织中ANGPT1、Smad9 mRNA表达情况,比较不同肿瘤转移侵袭行为患者癌组织中ANGPT1、Smad9 mRNA表达情况,分析ANGPT1、Smad9 mRNA表达与肺腺癌患者肿瘤转移侵袭行为的关系,统计肺腺癌患者1年生存率,比较不同ANGPT1、Smad9 mRNA表达量患者1年生存率的差异。结果:癌组织ANGPT1mRNA、Smad9 mRNA表达量均低于癌旁组织(2.45±0.26比11.18±0.93、4.23±0.31比7.58±0.65),差异有统计学意义(P<0.05)。不同临床分期、肿瘤直径、分化程度、有无淋巴结转移、胸膜侵犯患者癌组织ANGPT1、Smad9 mRNA基因表达量比较差异有统计学意义(P<0.05)。Spearman相关性分析结果表明,ANGPT1、Smad9 mRNA表达与肺腺癌临床分期、肿瘤直径、淋巴结转移、胸膜侵犯呈负相关(r=-0.517、-0.539、-0.606、-0.679,P<0.05),与分化程度呈正相关(r=0.628,P<0.05)。随访1年,1年生存率为72.41%。Kaplan-Meier曲线分析结果显示,癌组织ANGPT1mRNA、Smad9 mRNA低表达患者1年生存率低于高表达患者(P<0.05)。结论:癌组织ANGPT1、Smad9基因下调会加快肺腺癌肿瘤转移侵袭行为,上调两者基因表达可作为提高生存率的潜在途径。

Objective To explore the relationship between the expression of angiopoietin 1(ANGPT1)and Smadhomolog 9(Smad9)genes in cancer tissues and tumor metastasis,invasion behavior and prognosis in patients with lung adenocarcinoma.Methods Sixty patients with lung adenocarcinoma in Chengwu Hospital Affiliated to Shandong First Medical University from October 2018 to December 2019 were selected as the research objects.The expressions of ANGPT1 and Smad9 mRNA in cancer tissues and adjacent tissues were compared,as well as the expressions of ANGPT1 and Smad9 mRNA in cancer tissues of patients with different tumor metastasis and invasion behaviors.The relationship between ANGPT1 and Smad9 mRNA expression and tumor metastasis and invasion behavior of lung adenocarcinoma were analyzed,and the 1-year survival rate of patients with lung adenocarcinoma was calculated.The 1-year survival rate of patients with different ANGPT1 and SMAD9 mRNA expression levels were compared.Results The relative expression of ANGPT1 mRNA and Smad9 mRNA in cancer tissues were lower than those in adjacent tissues:2.45±0.26 vs.11.18±0.93,4.23±0.31 vs.7.58±0.65,the differences were statistically significant(P<0.05).There were significant differences in the relative gene expression of ANGPT1 and Smad9 mRNA in different clinical stages,tumor diameter,degree of differentiation,lymph node metastasis and pleural invasion(P<0.05).Spearman correlation analysis showed that the expression of ANGPT1 and Smad9 mRNA were negatively correlated with clinical stage,tumor diameter,lymph node metastasis and pleural invasion(r=-0.517,-0.539,-0.606,-0.679,P<0.05),and positively correlated with the degree of differentiation(r=0.628,P<0.05).The 1-year survival rate of 58 patients was 72.41%.Kaplan-Meier curve analysis showed that the 1-year survival rate of patients with low expression of ANGPT1 and Smad9 mRNA in cancer tissues were were lower than those in patients with high expression(P<0.05).Conclusions Down-regulation of ANGPT1 and Smad9 genes in cancer tissues will accelerate the metastasis and invasion behavior of lung adenocarcinoma.Up-regulating the expression of both genes can be a potential way to improve survival.