箭叶淫羊藿中一个新的黄酮苷类成分

A new flavonoid glycoside from Epimedium sagittatum

利用现代柱色谱技术,如大孔吸附树脂、硅胶、ODS、Sephadex LH-20和HW-40C柱色谱及半制备液相等对箭叶淫羊藿正丁醇部位化学成分进行分离纯化,得到14个黄酮类化合物。根据化合物的理化性质和波谱特征,确定其结构分别为3′-羟基宝藿苷-Ⅱ(1)、huazhongilexone-7-O-β-D-glucopyranoside (2)、kaempferol-3-O-α-L-rhamnoside (3)、宝藿苷-Ⅱ(4)、淫羊藿次苷-Ⅱ(5)、kaempferol 3,7-di-O-α-L-rhamnopyranoside (6)、(+)-aromadendrin (7)、kaempferol 3-O-(2-O-β-D-apiofuranosyl)-α-L-rhamnopyranoside (8)、箭藿苷A (9)、2″-O-rhamnosyl icariside-II (10)、apigenin-7-O-β-D-glucoside(11)、quercetin3-O-β-D-apiofuranoyl-(1→2)-α-L-rhamnopyranoside(12)、山柰酚(13)、淫羊藿苷(14)。其中化合物1为新化合物,化合物2、6~8、11和12为首次从箭叶淫羊藿中分离得到。

Fourteen flavonoids were isolated and purified from Epimedium sagittatum by various chromatography techniques such as macroporous adsorbent resin,silica gel,ODS,Sephadex LH-20,HW-40C and semipreparative HPLC.Their structures were identified by analysis of physicochemical properties and spectral data,and determined as 3′-hydroxy-baohuoside-Ⅱ(1),huazhongilexone-7-O-β-D-glucopyranoside(2),kaempferol-3-O-α-Lrhamnoside(3),baohuoside-Ⅱ(4),icariside-Ⅱ(5),kaempferol 3,7-di-O-α-L-rhamnopyranoside(6),(+)-aromadendrin(7),kaempferol 3-O-(2-O-β-D-apiofuranosyl)-α-L-rhamnopyranoside(8),sagittatoside A(9),2″-O-rhamnosyl icariside-II(10),apigenin-7-O-β-D-glucoside(11),quercetin 3-O-β-D-apiofuranoyl-(1→2)-α-L-rhamnopyranoside(12),kaempferol(13),icariin(14).Among them,compound 1 is a new compound,while compounds 2,6-8,11,and 12 were isolated from E.sagittatum for the first time.