新型重组人源弹性蛋白交联凝胶快速修复光损伤皮肤

A crosslinked hydrogel based on recombinant human elastin for rapid repair of photodamaged skin

光损伤是最常见的皮肤疾病,每年遭受皮肤光损伤的人次超过全球总人口的1/3.弹性蛋白是皮肤真皮层中的关键蛋白质,在维持皮肤形态中扮演着重要角色.弹性蛋白目前主要通过动物组织提取,它存在病毒传播隐患和容易降解等缺陷.本研究构建了一种新型的重组人源弹性蛋白及其交联凝胶,能够快速修复光损伤皮肤.该重组人源弹性蛋白由人弹性蛋白片段组成,具有与天然弹性蛋白类似的二级结构和可逆相变特性.该重组人源弹性蛋白经THPC交联形成凝胶,具有良好的机械强度.扫描电子显微镜结果表明,该重组弹性蛋白凝胶具有均匀分布的网孔结构.细胞实验表明,该凝胶无细胞毒性,并可以显著促进成纤维细胞的增殖.光损伤模型小鼠的动物实验表明,该凝胶可减轻光损伤小鼠的炎症反应,有效抑制表皮异常增生,并明显缩短皮肤愈合周期.本研究开发的高纯度重组人源弹性蛋白及其交联凝胶,对光损伤皮肤有显著的修复效果,在皮肤医学领域具有广泛的应用前景.

Photodamage is the most common skin disease,affecting more than a third of the global population each year.Elastin in dermis plays a critical role in maintaining skin shape,and its destruction is a prominent feature in skin photodamage.Currently,elastin is mostly derived from animal tissue,which has drawbacks such as virus transmission and easy degradation.We constructed a novel Recombinant Human Elastin(RHE)and its cross-linked hydrogel to rapidly repair photodamaged skin.RHE,composed of human elastin domains,preserves a similar secondary structure and reversible phase transition properties as native.RHE was cross-linked by THPC to form hydrogels with remarkable mechanical strength.Scanning electron microscopy results showed that the RHE hydrogel has a uniformly distributed mesh structure.Cell experiments indicated that the hydrogel had no cytotoxicity and significantly promoted the proliferation of fibroblasts.Animal experiments showed that the hydrogel significantly accelerated the healing of UVirradiated skin in mice,reducing the inflammatory response and inhibiting abnormal epidermal hyperplasia.The highly biocompatible and bioactive RHE and its cross-linked hydrogel provides an efficient treatment for photodamaged skin,which may have promising applications in dermatology and tissue engineering.