TNFAIP3在透明细胞肾细胞癌中的表达及临床意义

Expression and clinical significance of TNFAIP3 in clear cell renal cell carcinoma

目的:探讨TNFAIP3在透明细胞肾细胞癌(clear cell renal cell carcinoma, ccRCC)中的表达情况,并分析其表达与患者临床病理特征的相关性。方法:利用GEPIA2数据库分析TNFAIP3在ccRCC组织和正常肾脏组织中的差异表达,收集具备完整临床资料的97例ccRCC组织及对应的癌旁正常肾脏组织,采用免疫组织化学EnVision两步法检测TNFAIP3蛋白在ccRCC组织及对应癌旁正常肾脏组织中的表达,分析TNFAIP3蛋白的表达情况与患者年龄、性别、WHO/ISUP分级、肿瘤最大径及TNM分期等临床病理特征的关系。结果:GEPIA2数据库分析显示,与正常肾脏组织相比TNFAIP3在ccRCC组织中呈高表达(P<0.05),但TNFAIP3高表达ccRCC患者总生存时间和无瘤生存时间均显著高于TNFAIP3低表达ccRCC患者(P<0.05);TNFAIP3免疫组化检测证实,TNFAIP3在ccRCC组织中呈淡黄至棕褐色表达于细胞膜和细胞质,在ccRCC组织中高表达比例明显高于癌旁正常肾脏组织(P<0.05),TNFAIP3表达水平与ccRCC组织的WHO/ISUP肾细胞癌分级、肿瘤最大径、TNM分期、有无淋巴结转移临床参数有关(P<0.05),TNFAIP3高表达则提示较好的预后。结论:ccRCC组织中TNFAIP3的表达明显升高,而TNFAIP3高表达的ccRCC患者总生存期和无瘤生存期均好于TNFAIP3低表达的ccRCC患者,TNFAIP3参与ccRCC增殖、转移中的作用方式与分子调控机制则待进一步研究。

Objective:To investigate the expression of TNFAIP3 in clear cell renal cell carcinoma(ccRCC),and to analyze the correlation between TNFAIP3 expression and clinicopathological features of patients.Method:The GEPIA2 was used to analyze the differential expression of TNFAIP3 in ccRCC tissues and normal kidney tissues, with complete clinical information collection ccRCC group 97 cases and the corresponding tissue adjacent to carcinoma 97 cases.Immunohistochemistry EnVision two-step method was used to detect the expression of TNFAIP3 in ccRCC tissues and corresponding adjacent normal kidney tissues, and analyze the expression of TNFAIP3 with patients’ age, gender, WHO/ISUP grades, maximum tumor diameter, TNM staging and the relationship between the clinical pathological characteristics.Results:GEPIA2 analysis showed that compared with normal kidney tissue, TNFAIP3 was highly expressed in ccRCC tissue(P<0.05),but the overall survival and disease-free survival of ccRCC patients with high TNFAIP3 expression were significantly higher than those of ccRCC patients with low TNFAIP3 expression(P<0.05).Immunohistochemical detection of TNFAIP3 confirmed that TNFAIP3 was expressed in the cell membrane and cytoplasm from light yellow to brown in ccRCC tissues, and the proportion of high expression in ccRCC tissue is significantly higher than that in adjacent normal kidney tissue(P<0.05).The expression level of TNFAIP3 is related to the WHO/ISUP renal cell carcinoma grade, maximum tumor diameter, TNM stage, and clinical parameters of lymph node metastasis in ccRCC tissues(P<0.05).The high expression of TNFAIP3 indicates a better prognosis.Conclusion:The expression of TNFAIP3 in ccRCC tissues was significantly increased, but the overall survival and disease-free survival of ccRCC patients with high TNFAIP3 expression were better than those of ccRCC patients with low TNFAIP3 expression.However, the role and molecular regulation mechanism of TNFAIP3 involved in the proliferation and metastasis of ccRCC needs to be further studied.