茶树品种‘金牡丹’自然杂交后代遗传鉴定

Genetic Identification of the Natural Hybrid Progenies of ’Jinmudan’ Tea Varieties

为了对‘金牡丹’茶树自然杂交后代进行遗传鉴定,利用EST-SSR毛细管电泳荧光标记技术对65个金牡丹自然杂交后代进行研究。结果表明,28对SSR标记共扩增出192个等位片段,平均等位基因数(Na)、平均观测杂合度(Ho)及遗传多态信息量(PIC)分别为6.86、0.540、0.532。单亲基因型已知时的累积排除概率为0.999,说明选择的28对SSR标记位点具有高度的多态性和较高的排除概率,适用于遗传分析和个体的亲子鉴定。15个‘金牡丹’自然杂交后代的遗传鉴定结果表明,MD44、JMD45、JMD47、JMD32为早生绿茶类型;JMD51、JMD53为闽北肉桂乌龙茶类型;MD2、JMD56为闽南‘铁观音’乌龙茶类型;JMD24、JMD26、JMD29、JMD55、JMD59、JMD27、JMD61为‘黄棪’乌龙茶类型。

In order to analyze the genetic identification of the natural hybrid progenies of tea, EST-SSR capillary electrophoresis fluorescence maker technique was used to analyze 65 the natural hybrid progenies of ’Jinmudan’tea. The results showed that a total of 192 polymorphic alleles were detected by 28 SSR markers, the average number of alleles(Na), observed heterozygosity(Ho) and polymorphism information content(PIC) was 0.58, 0.58and 0.98, respectively. When the parental genotype was known, the combined exclusion probability was all reached 0.999, indicating that the 28 selected SSR marker sites had high polymorphism and high probability of exclusion, which were suitable for genetic analysis and paternity identification of individuals. The results of genetic identification showed that the 15 natural hybrid progenies of ’Jinmudan’ tea could be divided into 4 types,JMD44, JMD45, JMD47 and JMD32 were early green tea, JMD51 and JMD53 were ’Rougui’ oolong tea in northern Fujian, MD2 and JMD56 were ’Tieguanyin’ oolong tea in southern Fujian, JMD24, JMD26, JMD29,JMD55, JMD59 and JMD27 were ’Huangdan’ oolong tea.