白花蛇舌草的化学成分研究

Chemical Constituents from Hedyotis diffusa Willd.

为研究白花蛇舌草(Hedyotis diffusa)的化学成分,采用硅胶柱色谱、制备HPLC色谱等方法,从白花蛇舌草全草的70%乙醇提取物中分离得到9个化合物。根据理化性质及其波谱或光谱数据,结构分别鉴定为:豆甾醇(1)、β-谷甾醇(2)、rubiadin(3)、2,6-二羟基-1-甲氧基蒽醌(4)、β-谷甾醇-3-O-β-D-葡萄糖苷(5)、对香豆酸(6)、山奈酚(7)、槲皮素(8)和2-羟基-3-甲氧基-7-甲基蒽醌(9)。化合物4为新化合物;氯仿提取部位的Fr.4、Fr.4-2及Fr.4-4流分具有较强体外抗人子宫内膜癌Ishikawa细胞活性,其IC50值分别为52.8、78.1和27.5μg/mL。

To understand the chemical composition of Hedyotis diffusa, nine compounds were isolated from the 70% ethanol extract by column chromatography on silica gel and preparative high-performance liquid chromatography. Based on physicochemical properties and spectral data, their structures were identified as stigmasterol(1),β-sitosterol(2), rubiadin(3), 2,6-dihydroxy-1-methoxyanthraquinone(4), β-sitosterol-3-O-β-D-glucoside(5), pcoumaric acid(6), kaempferol(7), quercetin(8) and 2-hydroxy-3-methoxy-7-methylanthraquinone(9). Compound 4was a new compound. The Fr.4, Fr.4-2 and Fr.4-4 fractions in chloroform extract had strong activity against human endometrial carcinoma Ishikawa cells in vitro, with IC50of 52.8, 78.1 and 27.5 μg/mL, respectively.