ICP-MS法测定贡菊不同提取液中无机元素的浸出率

Leaching rate of inorganic elements in extracts of Gongju by ICP-MS

目的测定贡菊药材和贡菊不同提取液中无机元素的含量,比较不同提取方法所得贡菊提取液中无机元素浸出率的差异。方法根据含菊花中药制剂中菊花的常用提取方法(水煎煮、水热浸和70%乙醇回流)分别制备贡菊提取液,以Rh和Re两种元素作为内标,采用ICP-MS法测定贡菊不同提取液中20种无机元素的含量,计算不同元素的浸出率。结果贡菊水煎煮提取液中Al、Fe、Zn、Mo等13种无机元素的浸出率高于水热浸和70%乙醇回流提取液;70%乙醇回流提取液中Co、Cd、Cr浸出率高于水煎煮和水热浸提取液;除Ni、Ba、Se、Pb、As、Hg外,水热浸1.5 h提取液中无机元素浸出率高于水热浸1 h提取液。结论中药制剂中菊花的提取方法影响其提取液中无机元素的含量和浸出率。

Objective To determine the content of inorganic elements in Gongju medicinal materials and extracts of Gongju,and to compare the leaching rates of inorganic elements in the extracts of Gongju by different methods.Methods According to the common extraction methods of Chrysanthemi Flos in traditional Chinese medicine preparations containing Chrysanthemi Flos,Gongju was extracted by boiling water,hot dipping in water and refluxing with 70%ethanol.Rh and Re were used as the internal standards,and the content of 20 inorganic elements in the extracts was determined by ICP-MS.Results The leaching rates of 13 inorganic elements such as Al,Fe,Zn and Mo in water decoction extracts of Gongju were higher than those in hot water dip and 70%ethanol reflux extract,while the leaching rates of Co,Cd,and Cr in 70%ethanol reflux extract were higher than those in water decoction and hot water dip extract.Except for Ni,Ba,Se,Pb,As and Hg,the leaching rates of inorganic elements in the extract of 1.5 h hot water dip were higher than those of 1 h hot water dip.Conclusion The extraction methods of Chrysanthemi Flos in traditional Chinese medicine preparations affect the content and leaching rates of inorganic elements in its extracts.