单环刺螠体腔液细胞的流式分布及抗菌活性研究

Study on the Flow Distribution and Antibacterial Activity of Urechis unicinctus Coelomocytes

通过不连续密度梯度离心结合流式细胞术对单环刺螠体腔液细胞进行聚类,并对体腔液细胞抗菌活性进行测定。结果表明:利用流式细胞术将不连续密度梯度离心获得的Percoll各细胞层与全部的体腔液细胞对比,将单环刺螠体腔液细胞聚为3个亚群,分别为白色透明细胞、红色颗粒细胞和半颗粒细胞。体外试验发现,单环刺螠体腔液细胞具有一定的抑菌/杀菌能力,可以减少大肠杆菌引发的溶血现象,能通过呼吸爆发的方式抑制大肠杆菌的生长,抑菌率为22.36%;但其对枯草芽孢杆菌的抑制效果甚微。单环刺螠体腔液细胞分类及抗菌活性的研究有助于丰富单环刺螠基础生物学内容,完善单环刺螠的育苗、养殖的基础理论体系。

By the inconsequential density gradient centrifugation cominbed with flow cytometry,the coelomocytes were clustered,the antibacterial activity of coelomocytes was determined.The results showed that the cell layer obtained by Percoll discontinuous density gradient centrifugation was compared with all the coelomocytes by using flow cytometry,the coelomocytes were clustered into three subgroups,namely white hyalinocyte,red granulocyte and semi granular cells.In vitro experiment results showed that U.unicinctus coelomocytes had a certain bacteriological/bactericidal ability,which could reduce the hemolysis phenomenon caused by E.coli,and inhibit the growth of E.coli by the method of respiratory burst,with the antibacterial rate of 22.36%.The inhibition effect on B.subtilis was minimal.The classification and antibacterial activity study on U.unicinctus coelomocytes was helpful to enrich the basic biological content and improve the basic theoretical system of seedling and breeding of U.unicinctus.