白及粉末饮片品质评价研究

Quality evaluation of Bletilla striata powder decoction pieces

目的 探讨白及粉末饮片中浸出物、SO2及多糖含量测定方法。方法 参照2020年版中国药典,采用热浸或冷浸法分析不同批次白及粉末饮片浸出物含量,酸碱滴定法测定SO2残留量;采用硫酸-苯酚法测定样品中多糖含量。结果 10%乙醇冷浸法浸出物含量最高;酸碱滴定法重复性的相对标准偏差(RSD)为1.2%,加样回收率为80.89%~89.10%;16.5~99.0 mg/L葡萄糖浓度与吸光度呈良好的线性关系,线性方程为y=0.00889x-0.05159,r=0.9991。结论 该方法操作简单、准确度高、重复性好,为白及粉末饮片品质评价提供科学依据。

Objective To analyze the assays of extract, sulfur dioxide and polysaccharides of Bletilla striata powder decoction pieces(BsPDP). Methods According to the Chinese Pharmacopoeia(2020 ed), contents of extract, residual sulfur dioxide, and polysaccharides in different batches of BsPDP were measured by hot/cold extraction, acid-base titration, and sulfuric acid-phenol method, respectively. Results The extract content was higher using cold extraction with 10% ethanol.The relative standard deviation(RSD) was 1.2%, and recovery of spiked sample was 80.89%-89.10% using acid-base titration.16.5-99.0 mg/L glucose showed a good linear relationship with absorbance: regression equation y=0.00889x-0.05159,r=0.9991. Conclusion It is simple, accurate, and reproducible for evaluating the quality of BsPDP.