花生白藜芦醇和查尔酮合成酶基因的鉴定与表达分析

Identification and expression analysis of STS/CHS genes in peanut

花生含有丰富的白藜芦醇,具有很好的营养价值和保健功能。白藜芦醇合酶(STS)是白藜芦醇合成途径中的关键酶。本研究运用生物信息学方法,从花生基因组数据中鉴定出了50个STS基因,17个编码查尔酮合成酶CHS基因;两者同属聚酮化合物合成酶家族。对所鉴定基因组家族成员的染色体定位、系统发育、外显子-内含子结构、基因表达模式分析,结果表明大量STS基因在根、种皮、果皮、果针中高量表达,45个STS和8个CHS基因均应答紫外胁迫。本研究结果为花生STS/CHS基因家族成员的功能鉴定提供有价值的参考信息,为花生的品质改良提供理论依据与基因资源。

Peanut is rich in resveratrol, which has a very high nutritional value and health care function. Stilbene synthase(STS) is the key enzyme in resveratrol synthesis. In this research, we identified 50 peanut STS and 17 chalcone synthase(CHS) gene members from peanut genome by using multiple bioinformatics methods. Further,the chromosome location, phylogeny, exon-intron structure, and expression pattern of the gene family members was conducted, the results showed that most of the STS genes high expressed in root, testa, pericarp and gynophore, all the expressed 45 STS and 8 CHS genes response to UV treatment. This study laid a foundation for the functional identification of peanut STS/CHS genes, and provided theoretical basis and genetic resources for high-quality peanut breeding.