西药联合温和灸对甲状腺功能减退大鼠模型干预作用的机制研究

Mechanism of intervention of medicine combined with mild moxibustion on hypothyroidism rat model

目的:探究西药联合温和灸对甲减模型大鼠免疫因子及钠/碘共转运体(NIS)干预作用的机制。方法:丙硫氧嘧啶(PTU)溶液灌胃造模成功后,西药及西药加温和灸组以左甲状腺素钠混悬液60μg/kg体重灌胃,每日一次;西药加温和灸组在大椎、命门、脾俞、肾俞施温和灸,每穴10 min,每日1次,每6日休息1日;连续4周。模型组与西药组同方式、同频率固定;空白组不做处理。ELISA法检测血清中促甲状腺激素(TSH)、总甲状腺激素(TT4)、甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)、白细胞介素4(IL-4)、白细胞介素23(IL-23)的含量;免疫组化及R-T PCR法检测各组大鼠甲状腺组织中钠/碘共转运体(NIS)的含量及NISmRNA的表达量。结果:(1)与空白组比较,模型组大鼠血清中TSH、TPOAb、TGAb、IL-23含量上升,IL-4、TT4含量下降,甲状腺组织中NIS含量及NISmRNA表达量下降,差异有统计学意义(P<0.01);(2)与模型组比较西药组及西药加温和灸组大鼠血清中TSH、TPOAb、TGAb、IL-23含量下降,IL-4、TT4含量上升;甲状腺组织中NIS含量及NISmRNA表达量上升,差异有统计学意义(P<0.01);(3)与西药组相比西药加温和灸组大鼠甲状腺组织中NISmRNA表达量上升,差异有统计学意义(P<0.05);结论:西药加温和灸可以通过降低TPOAb、TGAb、及IL-23的含量,升高IL-4的含量;增加NIS的含量及NISmRNA的表达量,干预甲减模型大鼠。

Objective:To explore the intervention effect of medicine combined with mild moxibustion on immune factor and Na/I symporter(NIS)in hypothyroidism rat model.Methods:The model was successfully made by intragastric administration of propylthiouracil(PTU)solution medicine and medicine combined with mild moxibustion groups were given levothyroxine sodium suspension 60μg/kg body weight by gavage,once a day.In the medicine combined with mild moxibustion group,mild moxibustion was applied in“Dazhui”,“Mingmen”,“Pishu”,“Shenshu”,and 10 minutes per point,once a day,and one day off every six days;four weeks in a row.The model group and medicine was fixed in the same way as the medicine combined with mild moxibustion group.The blank group received no treatment.The contents of thyrotropin-releasing hormone(TSH),tatalthyroxine(TT4),thyroid peroxidase antibody(TPOAb),thyroglobulin antibodies(TGAb),Interleukin-4(IL-4)and Interleukin-23(IL-23)in serum were determined by Enzyme Linked Immunosorbent Assay(ELISA).The content of NIS and the expression level of NISmRNA in thyroid tissues of each group were detected by immunohistochemistry and real-time polymerase chain reaction(R-T PCR).Results:Compared with the blank group,the contents of TSH,TPOAb,TGAb and IL-23 in the serum of rats in the model group were increased,the contents of IL-4 and TT4 were decreased,and the contents of NIS and NISmRNA in thyroid tissue were decreased,with statistical significance(P<0.01).Compared with model group,the contents of TSH,TPOAb,TGAb and IL-23 in serum of medicine group and medicine combined with mild moxibustion group were decreased,while the contents of IL-4 and TT4 were increased;the NIS content and NISmRNA expression in thyroid tissues were increased,and the differences were statistically significant(P<0.01).Compared with the medicine group,NISmRNA expression in thyroid tissues of medicine combined with mild moxibustion groups was increased,and the difference was statistically significant(P<0.05).Conclusion:Medicine combined with mild moxibustion can decrease the contents of TPOAb,TGAb and IL-23,and increase the content of IL-4,increasing the content and expression of NIS to interfere with the hypothyroidism rat model.