紫癜性肾炎外周血CD25的表达及意义

Expression and significance of CD25 in peripheral blood in purpura nephritis

目的探讨外周血T细胞表面和血清CD25表达水平与儿童过敏性紫癜(HSP)并发肾炎的相关性,为HSP病情的评估提供帮助。方法收集中国医科大学附属第一医院2020年1月至2021年11月收治的HSP患儿40例作为研究组,根据肾脏受累情况再分为紫癜性肾炎(HSPN,18例)组与非紫癜性肾炎(NHSPN,22例)组。同期健康体检儿童25名作为对照组(HD)。采用流式细胞仪检测外周血T细胞中CD25的表达。酶联反应吸附(ELISA)法测定血清CD25水平。比较组间差异及相关性。结果与对照组相比,HSP患儿外周血中CD25在CD3^(+)总T细胞[HD(2.19±0.85)%、HSP(1.43±0.78)%]、CD3^(+)CD4^(+)T细胞[HD(3.82±2.18)%、HSP(2.40±0.95)%]、CD3^(+)CD4^(-)T细胞[HD(1.90±0.51)%、HSP(1.25±0.35)%]上的表达均减少(P<0.05)。HSPN患儿外周血CD3^(+)CD4^(+)T细胞中CD25的表达[NHSPN(2.62±1.03)%、HSPN(2.11±0.78)%]和血清CD25水平[NHSPN(649.0±346.5)pg/mL、HSPN(407.0±141.0)pg/mL]较NHSPN患儿下降(P<0.05)。HSP患儿血清CD25水平与Ig A水平呈负相关(r=-0.5060,P<0.05),而外周血T细胞中CD25的表达与血清Ig A水平相关性差异无统计学意义。结论外周血T细胞CD25的表达和血清CD25的水平与HSP疾病发生和肾脏受累情况相关,有助于疾病的评估。

Objective To investigate the relationship of CD25 expression in peripheral blood T cells andserum CD25 level with nephritis with Henoch-Schonlein purpura(HSP)in children,in order to help evaluate HSP.Methods Forty children diagnosed with HSP were collected as study group and divided into two groups according torenal involvement:HSPN group(18 cases)and NHSPN group(22 cases).During the same period,25 healthy childrenwere taken as control group.The expression of CD25 in peripheral blood T cells was detected by flow cytometry.SerumCD25 levels were determined by enzyme-linked adsorption(ELISA).The differences and relationship between the twogroups were compared.Results Compared with the control group,the expression of CD25 in peripheral blood of HSPchildren were decreased in CD3^(+)total T cells[HD(2.19±0.85)%、HSP(1.43±0.78)%],CD3^(+)CD4^(+)T cells[HD(3.82±2.18)%、HSP(2.40±0.95)%]and CD3^(+)CD4^(-)T cells[HD(1.90±0.51)%、HSP(1.25±0.35)%](P<0.05).The expressionof CD25 in peripheral blood CD3^(+)CD4^(+)T cells[NHSPN(2.62±1.03)%、HSPN(2.11±0.78)%]and the level of serumCD25[NHSPN(649.0±346.5)pg/mL、HSPN(407.0±141.0)pg/mL]in HSPN children were lower than those in NHSPNchildren(P<0.05).Serum CD25 level was negatively correlated with IgA level in HSP children(r=-0.5060,P<0.05),butwas the expression of CD25[NHSPN(649.0±346.5)pg/mL、HSPN(407.0±141.0)pg/mL]in peripheral blood T cells wasnot related To IgA level.Conclusion The expression of CD25 in peripheral blood T cells and the level of serum CD25are closely related to the developrnent and renal involvement of children with HSP,which is helpful for the evaluation ofthe disease.