基于网络药理学和分子对接探讨瑶山甜茶治疗2型糖尿病的作用机制

Mechanism of Rubus suavissmus S.Lee in Intervention of Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking Method

为了探讨瑶山甜茶治疗2型糖尿病的作用机制,应用高脂高糖饲料喂养结合链脲佐菌素(STZ)复制2型糖尿病大鼠模型,观察大鼠血糖和胰腺组织的变化;运用系统药理学方法查找并筛选瑶山甜茶的活性成分靶点及2型糖尿病相关基因,并进行富集分析以预测其可能的信号通路,对筛选所得核心成分与核心靶点进行分子对接验证,探究其分子机制。结果表明:瑶山甜茶显著降低糖尿病模型大鼠血糖(P<0.05),同时改善其胰岛细胞损伤。筛选出瑶山甜茶干预2型糖尿病的有效成分59个,核心靶点17个。KEGG通路富集分析发现癌症信号通路、FOXO信号通路和HIF-1信号通路与2型糖尿病密切相关。分子对接表明黄芪苷、咖啡酸、槲皮素可能是瑶山甜茶治疗2型糖尿病抗血糖的物质基础,与调控MAPK1、EGFR、SRC、AKT1、PIK3R1、PTPN11信号通路有关。

To explore the mechanism of Rubus suavissmus S.Lee in the intervention of type 2 diabetes mellitus,type 2 diabetic rats were fed with high-fat and high-sugar diet combined with streptozotocin(STZ)to observe the changes of blood glucose and pancreatic tissue.The active ingredient targets and type 2 diabetes mellitus related genes of Rubus suavissmus S.Lee were searched and screened by systematic pharmacology method,and enrichment analysis was conducted to predict their possible signaling pathways.Molecular docking verification was conducted between the screened core ingredients and the core target,and the molecular mechanism was explored.The results showed that Rubus suavissmus S.Lee significantly reduced blood sugar in diabetic model rats(P<0.05),and at the same time improved its islet cell damage.KEGG pathway enrichment analysis showed that cancer signaling pathway,FOXO signaling pathway and HIF-1 signaling pathway were closely related to type 2 diabetes mellitus.The molecular docking results showed that baicalin,quercetin and caffeic acid may be the material basis for the anti-glycemic treatment of type 2 diabetes by Rubus suavissmus S.Lee,which was related to the regulation of MAPK1,EGFR,SRC,AKT1,PIK3R1 and PTPN11 signaling pathways.