草莓病毒1山东分离物全基因组分析

Genome Sequence Analysis of Strawberry Virus 1 from Shandong Province,China

草莓病毒1(strawberry virus 1,StrV-1)属于弹状病毒科(Rhabdoviridae)细胞质弹状病毒属(Cytorhabdovirus),可侵染草莓。利用高通量测序(high-throughput sequencing,HTS)结合RT-PCR和RACE技术从采自山东省烟台市乳山市草莓种植基地的草莓品种‘全明星’上获得了StrV-1山东分离物(StrV-1-CN)的基因组全长(GenBank登录号:MW795715)。该分离物基因组全长为14051 nt,3ʹ和5ʹ非编码区序列分别为181和767 nt,其负义链含有9个开放阅读框(open reading frame,ORF),从3ʹ到5ʹ端分别编码N、Pʹ、P、P3、M、G、P6、P7和L等9个蛋白。序列分析表明,StrV-1-CN与已报道的StrV-1分离物基因组全长核苷酸序列一致性为77%~88%。系统发育分析结果表明,StrV-1-CN与捷克分离物B和1/2017聚在一个分支,亲缘性最近。

Strawberry virus 1(StrV-1,genus Cytorhabdovirus,family Rhabdoviridae)was a new pathogen that infecting strawberry.The complete genome sequence of StrV-1 identified in strawberry(Fragaria ananassa Duch.‘All Star’)from Shandong Province,China(StrV-1-CN)was determined and submitted to the GenBank database under accession No.MW795715.In the negative strand,the 14051-nucleotide-long viral genome with 181 and 767 nt in the 3ʹand 5ʹUTR,and contains nine open reading frames(ORFs)in the canonical order 3ʹ-N-Pʹ-P-P3-M-G-P6-P7-L-5ʹ.Pairwise comparisons showed that StrV-1-CN shared 77%–88%sequence identity with StrV-1 isolates.Phylogenetic analysis based on the complete genome sequence of StrV-1 isolates showed that StrV-1-CN grouped with StrV-1 isolate B and isolate 1/2017 in a subcluster.