摘要
2020年,在云南中医药大学校园发现叶片表现褪绿斑点、坏死环斑症状的鸢尾植株。用电子显微镜在病叶汁液中观察到典型的正番茄斑萎病毒属(Orthotospovirus)病毒粒子。在用感病叶片汁液摩擦接种的普通烟、本生烟上观察到系统坏死斑症状,中国辣椒(PI152225)的接种叶片出现局部坏死斑,但无系统侵染。为进一步明确病原,用RT-PCR扩增番茄斑萎病毒(tomato spotted wilt orthotospovirus,TSWV)的NSs和N基因,序列测定发现,分别与分离自云南番茄和旱金莲的TSWV分离物具有最高的氨基酸序列相似性(99.79%和100%)。结果表明引起鸢尾褪绿斑点病的病原是番茄斑萎病毒。
In 2020,Iris tectorum Maxim plants exhibiting chlorotic spot and necrotic ringspot symptoms were observed on campus in Yunnan University of Chinese Medicine in Kunming City,Yunnan Province.The typical virions of orthotospoviruses were observed by electron microscope.The systemic necrotic spots symptoms were found on the plants of Nicotiana tabacum and N.benthamiana inoculated mechanically with the sap of the symptomatic I.tectorum Maxim leaves.The inoculated leaves of Capsicum chinense Jacquin PI152225 showed the local lethal spots,whilst no systemic infection were detected.To further confirm the pathogen,RT-PCR were performed to amplify the NSs and N genes of tomato spotted wilt orthotospovirus(TSWV),respectively.Sequence analysis showed the obtained sequences had the highest identities(99.79%and 100%)with NSs and N genes of TSWV isolates infecting tomato and Tropaeolum majus in Yunnan Province.The results indicated TSWV infection causes the chlorotic spots and necrotic ringspots sympt,oms in I.tectorum.
作者
忽靖宇
阙开娟
缪田丽
吴少政
王田田
张磊
董鲜
季鹏章
董家红
HU Jingyu;QUE Kaijuan;MIAO Tianli;WU Shaozheng;WANG Tiantian;ZHANG Lei;DONG Xian;JI Pengzhang;DONG Jiahong(School of Chinese Materia Medica and Yunnan,Key Laboratory of Southern Medicinal Resource,Yunnan University of Chinese Medicine,Kunming 650500,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第1期170-176,共7页
Acta Horticulturae Sinica
基金
云南中医药大学高层次人才科研项目(30270104870)
国家自然科学基金项目(31371919)。
关键词
鸢尾
番茄斑萎病毒
N基因
NSs基因
Iris tectorum
tomato spotted wilt orthotospovirus
N gene
NSs gene
