miR-876-3p在高氧诱导新生大鼠支气管肺发育不良模型中的表达分析

The expression of miR-876-3p based on a rat model of bronchopulmonary dysplasia induced by hyperoxia

目的:通过高氧诱导建立新生大鼠支气管肺发育不良(BPD)模型,动态观察其肺组织病理改变并检测肺组织中miR-876-3p的表达,探讨miR-876-3p在BPD发生发展中的作用。方法:选取新生SD大鼠80只按随机数字表法随机分为高氧组(FiO 260%)及空气组(FiO 221%)。分别于生后第1、7、14、21天取大鼠肺组织标本,观察肺组织病理变化,应用实时荧光定量PCR(qRT-PCR)技术检测miR-876-3p的表达。结果:生后21 d内,随高氧暴露时间延长,高氧组大鼠较空气组大鼠一般生长情况差,体重低[14 d:(35.46±1.62)g比(37.08±1.25)g;21 d:(51.92±1.83)g比(58.87±2.43)g],差异有统计学意义(P<0.05)。生后第14、21天,高氧组大鼠辐射状肺泡计数较空气组大鼠明显减少,差异有统计学意义(P<0.05);生后第7、14、21天,空气组大鼠与高氧组大鼠肺泡间隔厚度相比均偏低,差异有统计学意义(P<0.05)。高氧组miR-876-3p的表达在生后第7、14、21天较同时间点空气组明显降低[7 d:(14.97±1.13)比(16.64±0.89);14 d:(11.92±0.71)比(16.85±0.79);21 d:(11.39±0.79)比(17.52±1.17)],差异均有统计学意义(P均<0.01)。结论:通过高氧诱导可构建新生大鼠新型BPD模型,该模型中miR-876-3p的表达水平降低,其差异性表达可能在BPD的发生发展中有一定作用。

Objective To establish a neonatal rat bronchopulmonary dysplasia(BPD)model induced by hyperoxia,to detect the expression of miR-876-3p in the lung tissue,and to analyze the role of miR-876-3p in the occurrence and development of BPD,so as to provide a theoretical basis for the pathogenesis,prevention and treatment of BPD.Methods Eighty newborn SD rats were randomly divided into hyperoxia group(FiO260%)and air group(FiO221%).Lung tissue samples were taken on the 1st,7th,14th and 21st day after birth,the pathological changes of lung tissue were observed.Quantitative real-time PCR technique was used to detect the expression level of miR-876-3p.Results Within 21 days after birth,with the prolongation of hyperoxia exposure time,the general growth of rats in hyperoxia group were lower than those in air group[14 d:(35.46±1.62)g vs.(37.08±1.25)g;21 d:(51.92±1.83)g vs.(58.87±2.43)g](P<0.05).On the 14th and 21st day after birth,the radial alveolar counts in lung tissue of rats in hyperoxia group were significantly reduced compared with those in air group(P<0.05).On the 7th,14th and 21st day after birth,the alveolar septal thickness of rats in air group were lower than those in hyperoxia group(P<0.05).The expression level of miR-876-3p in hyperoxia group decreased gradually and was significantly lower on the 7th,14th and 21st day compared with air group at the same time points[7 d:(14.97±1.13)vs.(16.64±0.89);14 d:(11.92±0.71)vs.(16.85±0.79);21 d:(11.39±0.79)vs.(17.52±1.17)],and the differences were all statistically significant(P all<0.01).Conclusion In this study,a new BPD model of neonatal rats can be induced by hyperoxia and the expression level of miR-876-3p in this model is decreased.The differential expression level of miR-876-3p may play a role in the occurrence and development of BPD.