黄芩总黄酮提取工艺优化及其抗氧化活性

Extraction Process Optimization and Antioxidant Activity of Flavonoids from Scutellaria baicalensis

为优化黄芩(Scutellaria baicalensis)总黄酮提取工艺并对其抗氧化活性进行研究,采用单因素试验,研究乙醇浓度、料液比、浸泡时间、提取温度和提取时间等因素对黄芩总黄酮提取率的影响,采用响应面试验优化总黄酮提取工艺,通过建立数学模型预测得到最佳工艺条件,并测定黄芩总黄酮提取物对DPPH自由基的清除率。结果表明,最优提取工艺为乙醇浓度58.50%(体积分数)、料液比1∶47 g/mL、浸泡45 min、提取温度65.5℃、提取时间2 h,提取率为10.52%。采用最优工艺提取出的黄芩总黄酮提取物对DPPH自由基有较好的清除效果,浓度为100 mg/mL的黄芩总黄酮提取物对应的清除率为81.25%。优化后的黄芩总黄酮提取工艺稳定可行,提取出的总黄酮提取物有较好的抗氧化活性。

In order to optimize extraction process of total flavonoids from Scutellaria baicalensis and study antioxidant activity of total flavonoids,effects of ethanol concentration,solid-liquid ratio,extraction temperature,soaking time and extraction time on extraction rate of total flavonoids from S.baicalensis were studied by single factor test.Extraction process of total flavonoids was optimized by response surface experiment.The optimal process conditions were predicted by establishing mathematical model,and the scavenging rates of total flavonoid extracts from S.baicalensis for DPPH free radicals were tested.Results showed that the optimal extraction process was ethanol concentration 58.50%(volume fraction),solid-liquid ratio 1∶47 g/mL,soaking for 45 min,extraction temperature 65.5℃and extracting 2 h with extraction rate of 10.52%.Total flavonoid extract extracted by the optimal process had good scavenging effect on DPPH free radicals,and corresponding scavenging rate of S.baicalensis total flavonoid extract with concentration of 100 mg/mL was 81.25%.The optimal extraction process of total flavonoids from S.baicalensis was stable and feasible,and extracted total flavonoid extracts had good antioxidant activity.