基于文献数据库的6-OHDA帕金森病大鼠模型特点分析及在中药研究中的应用

Analysis on the characteristics of 6-OHDA induced rat model of Parkinson’s disease based on literature data base and the application in the study of traditional Chinese medicine

目的分析6-OHDA诱导的大鼠PD模型的品系选择、性别、造模要素、模型评价方法以及在抗PD中药研究中的应用情况,以期为6-OHDA模型在帕金森研究中的应用提供参考。方法查阅近20年来中国知网(CNKI)和PubMed中应用6-OHDA建立PD大鼠模型的相关文献,分析6-OHDA诱导的大鼠PD模型的品系选择、性别体重、造模要素、模型评价方法以及在抗PD中药研究中的应用情况。结果6-OHDA诱导的大鼠PD模型多选用雄性SD或Wistar大鼠,造模时注射部位多为单侧黑质、内侧前脑束或纹状体,多在造模4周后检测阿扑吗啡诱导旋转实验、脑组织形态学、分子生物学、生化指标以判断模型成功率及防治PD药物的有效性。结论本文通过对6-OHDA诱导的大鼠PD模型的品系选择、性别、体重、造模要素、模型评价方法以及在抗PD中药研究中的应用情况进行分析,为PD模型的造模和应用提供参考。

Objective To analyze the strain selection,sex,modeling key points,evaluation method of 6-hydroxydopamine(6-OHDA)induced Parkinson’s disease(PD)model in rats and its application on the screening of traditional Chinese medicine in the treatment of Parkinson’s disease.Methods Recent literatures related to 6-OHDA models in the CNKI and PubMed databases in 20 years were collected.The strains of rats,the weight,sex,modeling key points,evaluation method were analyzed,along with the application of 6-OHDA model in anti-PD traditional Chinese medicine screening.Results Male SD or Wistar rats are mostly selected as PD models in rats induced by 6-OHDA.The injection site is usually unilateral substantia nigra,medial forebrain bundle or striatum.Apomorphine induced rotation experiment,brain histomorphology,molecular biology and biochemical indicators are usually detected 4 weeks after modeling to determine the success rate of the model and the effectiveness of drugs for prevention and treatment of PD.Conclusions This study provides a useful reference for the use of 6-OHDA induced PD model regarding the strain selection,sex,modeling key points,evaluation method and the application on the screening of traditional Chinese medicine in the treatment of PD.