不同抗凝剂配比和标本放置时间对血液细胞学检验结果的影响

Effect of different anticoagulant ratios and specimen storage time on blood cytology test results

目的研究不同抗凝剂配比和标本放置时间对血液细胞学检验结果的影响。方法选择2021年12月—2022年10月在聊城市第二人民医院进行血液细胞学检验的46例患者作为研究对象,收集血液样本,对使用不同抗凝剂配比(1︰10000、1︰5000)和放置不同时间(30 min、60 min、120 min)的血液样本进行血液细胞学检测[包括红细胞计数(RBC)、白细胞计数(WBC)、淋巴细胞比例(LYM%)、血红蛋白(Hb)、血小板计数(PLT)、红细胞分布宽度(RDW)],观察和比较检验结果。结果抗凝剂配比1︰10000组的RBC、WBC、LYM%、Hb、PLT均明显高于抗凝剂配比1︰5000组[RBC(×10^(12)/L):5.54±0.42比4.06±0.32,WBC(×10^(9)/L):9.84±1.07比6.41±2.13,LYM%:(5.56±0.49)%比(4.31±0.23)%,Hb(g/L):154.63±12.16比105.95±9.76,PLT(×10^(9)/L):186.77±25.73比132.11±22.64,均P<0.05]。放置120 min组的RBC和RDW均明显高于放置60 min、30 min组[RBC(×10^(12)/L):5.87±0.51比5.00±0.42、4.08±0.35,RDW:(19.76±4.07)%比(16.23±3.44)%、(13.58±2.92)%,均P<0.05],LYM%明显低于放置60 min、30 min组[(3.15±0.10)%比(4.06±0.12)%、(4.30±0.22)%,均P<0.05];放置120 min组的WBC、Hb、PLT均明显低于放置60 min组[WBC(×10^(9)/L):6.20±0.58比8.70±0.92,Hb(g/L):127.41±10.89比133.67±12.54,PLT(×10^(9)/L):151.95±20.70比182.06±22.63,均P<0.05],Hb、PLT均明显高于放置30 min组[Hb(g/L):127.41±10.89比108.81±10.22,PLT(×10^(9)/L):151.95±20.70比142.49±19.17,均P<0.05]。结论血液细胞学检验结果受抗凝剂配比和标本放置时间的影响,临床检验中应加强血液细胞学检验质量控制。

Objective To investigate the effect of different anticoagulant ratios and specimen storage time on the results of blood cytology test.Methods The 46 patients in Liaocheng Second People’s Hospital from December 2021 to October 2022 were selected.The blood samples with different anticoagulant ratios(1︰10000 and 1︰5000)and stored for different time(30,60 and 120 minutes)were examined for blood cytology[red blood cell count(WBC),white blood cell count(WBC),lymphocyte ratio(LYM%),hemoglobin(Hb),platelet count(PLT)and red blood cell distribution width(RDW)].The test results were observed and compared.Results The levels of RBC,WBC,LYM%,Hb and PLT with 1︰10000 ratio were higher than those with 1︰5000 ratio[RBC(×10^(12)/L):5.54±0.42 vs.4.06±0.32,WBC(×10^(9)/L):9.84±1.07 vs.6.41±2.13,LYM%:(5.56±0.49)%vs.(4.31±0.23)%,Hb(g/L):154.63±12.16 vs.105.95±9.76,PLT(×10^(9)/L):186.77±25.73 vs.132.11±22.64,all P<0.05].The levels of RBC and RDW at 120 minutes were higher than those at 60 minutes and 30 minutes[RBC(×10^(12)/L):5.87±0.51 vs.5.00±0.42,4.08±0.35,RDW:(19.76±4.07)%vs.(16.23±3.44)%,(13.58±2.92)%,all P<0.05],and the level of LYM%was lower than those at 60 minutes and 30 minutes[(3.15±0.10)%vs.(4.06±0.12)%,(4.30±0.22)%,both P<0.05].The levels of WBC,Hb and PLT after 120 minutes of placement were lower than those after 60 minutes[WBC(×10^(9)/L):6.20±0.58 vs.8.70±0.92,Hb(g/L):127.41±10.89 vs.133.67±12.54,PLT(×10^(9)/L):151.95±20.70 vs.182.06±22.63,all P<0.05],but Hb and PLT were higher than those at 30 minutes[Hb(g/L):127.41±10.89 vs.108.81±10.22,PLT(×10^(9)/L):151.95±20.70 vs.142.49±19.17,both P<0.05].Conclusion The results of blood cytology test could be affected by the ratio of anticoagulant agent and time of specimen storage,so the quality control of blood cytology test would be strengthened in the clinical test.