单细胞转录组测序技术发展及其在甘薯中的应用

Development of single-cell RNA-seq technology and its application in sweet potato

单细胞转录组测序(single-cell RNA sequencing,scRNA-seq)技术是在单个细胞水平通过对mRNA进行高通量测序研究其整体水平基因表达情况的一项新技术。本研究概述了scRNA-seq技术的发展历程和创新;详细介绍了scRNA-seq技术的单细胞捕获/分选、反转录/PCR扩增、建库测序和测序数据基础分析等步骤,以及在植物单细胞图谱、非生物胁迫响应机制、细胞分化发育、转录因子调控网络等研究中的应用;剖析了该技术在甘薯中单细胞图谱、分化、抗逆和基因方面的应用潜力;最后对该技术的应用进行了展望。

Single-cell RNA-seq(scRNA-seq)technology is new to study overall gene expression at the single cell level by high-throughput sequencing of mRNA.This paper summarized the development and innovation of single cell transcriptome sequencing technology.The steps of single cell capture or sorting,reverse transcription/PCR amplification,database construction and sequencing data basic analysis of scRNA-seq technology were introduced in detail,and its application in the study of scRNA-seq technology in plant single cell map,abiotic stress response mechanism,cell differentiation and development,and transcription factor regulatory network were introduced.The application potential of single cell map,differentiation,stress resistance and gene in sweet potato was also analyzed.Finally,the application of this technology was prospected.