sclerostin在2型糖尿病伴牙周炎大鼠牙槽骨骨重建过程中的表达及意义

Expression and significance of sclerostin in the process of alveolar bone reconstruction in type 2 diabetic rats with periodontitis

目的:观察2型糖尿病(T2DM)伴牙周炎大鼠牙槽骨骨重建过程中骨硬化蛋白(sclerostin)的表达。方法:将54只SD大鼠随机分为健康组、牙周炎组、T2DM伴牙周炎组,每组各18只。牙周炎组建立牙周炎大鼠模型,T2DM伴牙周炎组先建立T2DM模型,再建立牙周炎模型。腹腔注射STZ后1、5、10 d,检测糖代谢指标。结扎后8周,检测牙周指标。建模成功后1、3、6个月,免疫组织化学染色检测牙槽骨组织中sclerostin的表达。采用SPSS 21.0软件包对数据进行统计学分析。结果:与未建模大鼠相比,T2DM建模大鼠腹腔注射STZ后1、5、10 d空腹血糖(FBG),腹腔注射STZ后10 d空腹胰岛素(FINS)及胰岛素抵抗指数(HOMA-IR)均显著升高(P<0.05)。与未建模大鼠相比,牙周炎建模大鼠牙龈出血指数(SBI)、菌斑指数(PLI)、探针深度(PD)均显著增加(P<0.05)。与健康组相比,牙周炎组、T2DM伴牙周炎组建模成功后1、3、6个月牙槽骨组织中sclerostin表达显著增加(P<0.05),且T2DM伴牙周炎组显著高于牙周炎组(P<0.05)。与建模成功后1个月相比,牙周炎组、T2DM伴牙周炎组建模成功后3、6个月牙槽骨组织中sclerostin表达显著增加(P<0.05)。与建模成功后3个月相比,牙周炎组、T2DM伴牙周炎组建模成功后6个月牙槽骨组织中sclerostin表达显著减少(P<0.05)。结论:sclerostin在牙周炎中表达增加,且合并T2DM进一步上调sclerostin的表达,但在骨重建过程中逐渐下调。

PURPOSE:To observe the expression of sclerostin in the process of alveolar bone reconstruction in rats with type 2 diabetes(T2DM) and periodontitis.METHODS:Fifty-four SD rats were randomly divided into control group,periodontitis group,and T2DM with periodontitis group with 18 rats in each group.The periodontitis group included a rat model with periodontitis,and the T2DM with periodontitis group included a T2DM model first,and then established a pe-riodontitis model.The glucose metabolism indicators were detected at 1,5,and 10 days after intraperitoneal injection of STZ.The periodontal indexes were detected at 8 weeks after ligation.The expression of sclerostin in alveolar bone tissue were detected by immunohistochemical staining 1,3,6 months after successful modeling.SPSS 21.0 software package was used for data analysis.RESULTS:Compared with unmodeled rats,fasting blood glucose(FBG) at 1,5,and 10 days after intraperitoneal injection of STZ,fasting serum insulin(FINS) and homeostasis model assessment for insulin resistance(HOMA-IR) at 10 days after intraperitoneal injection of STZ in T2DM model rats were increased(P<0.05).Compared with unmodeled rats,the gingival bleeding index(SBI),plaque index(PLI) and probe depth(PD) in periodontitis model rats were increased(P<0.05).Compared with control group,the expression of sclerostin in the alveolar bone tissue at 1,3,and 6months after successful modeling in periodontitis group and T2DM with periodontitis group was increased(P<0.05),which was significantly higher in T2DM with periodontitis group than in periodontitis group(P<0.05).Compared with 1 month after successful modeling,the expression of sclerostin in the alveolar bone tissue at 3,6 months after successful modeling in periodontitis group and T2DM with periodontitis group was increased(P<0.05).Compared with 3 months after successful modeling,the expression of sclerostin in the alveolar bone tissue at 6 months after successful modeling in periodontitis group and T2DM with periodontitis group was decreased(P<0.05).CONCLUSIONS:The expression of sclerostin is increased in periodontitis,and the expression of sclerostin with T2DM is further up-regulated,but it is gradually down-regulated during the process of bone remodeling.