纳米氧化锌颗粒对滨海湿地微生物群落的影响

Effect of ZnO nano particles on bacterial community in coastal wetlands

利用滨海湿地模拟系统研究纳米氧化锌颗粒(ZnO NPs)对滨海湿地周期淹水区和长期淹水区微生物群落组成和多样性的影响,采用16S rRNA扩增子测序查明发生显著性变化的微生物。结果表明:ZnO NPs暴露下微生物群落的相对丰度和多样性均受到不同程度的影响;ZnO NPs质量浓度的大小是驱动滨海湿地环境中微生物群落相对丰度、β多样性变化的重要原因。随着ZnO NPs质量浓度的增大,ZnO NPs团聚后增大的纳米材料颗粒粒径减弱了对微生物群落的影响;β多样性在ZnO NPs质量浓度为80 mg/L时最低;周期淹水区的微生物群落受到的影响比长期淹水区微生物群落受到的影响小,不同淹水条件导致出现不同分类水平的显著差异细菌;周期淹水区和长期淹水区不同的环境条件(如溶解氧、有机质)导致微生物群落丰度、多样性受ZnO NPs的影响表现不同。

The effects of ZnO nano particles(ZnO NPs) on the composition and diversity of microbial communities in the periodic and long-term submerged areas of coastal wetlands were studied with coastal wetland simulation systems, and the microbes with significant changes were identified by 16S rRNA amplicon sequencing. The results showed the relative abundances and diversities of microbial communities were varyingly affected under the ZnO NPs exposure. ZnO NPs concentration was an important factor that motivated the changes of relative abundances and β diversities of microbial communities in coastal wetland environment. With the increase of the concentration of ZnO NPs, the increased particle size of NPs after agglomeration weakened the impact on microbial community. β diversity was the lowest when the mass concentration of ZnO NPs was 80 mg/L. The influence on microbial community in the periodic submerged area was smaller than that in the long-term submerged area and submerging conditions lead to significantly different bacteria with different classification levels. Different environmental conditions(e.g. dissolved oxygen and organic matter) in periodic submerging area and long-term submerging area led to different effects of ZnO NPs on microbial abundance and community diversity.