基于PMA-qPCR方法检测兽用消毒剂对非洲猪瘟病毒的灭活效率

Inactivation efficiency of various disinfectants against African swine fever virus evaluated by PMA-qPCR

为评估规模化猪场生物安全防控水平,拟建立非洲猪瘟病毒(African swine fever virus,ASFV)感染性检测方法,并利用该方法检测兽用消毒剂对病毒的灭活效果。使用不同种类的消毒剂处理ASFV,通过PMA-qPCR评估消毒剂对ASFV的灭活效果和筛选消毒剂的最佳处理方式。PMA-qPCR结果显示,ASFV对不同类型的消毒剂表现出不同的敏感性,对过硫酸氢钾复合物溶液敏感性最高,该消毒剂按0.5%稀释,20 min内即可有效灭活ASFV。研究结果对生物安全的消毒环节具有一定的参考意义。

In order to evaluate the biosecurity prevention and control level of large-scale pig farms,this study intends to establish a method to detect the infectivity of African swine fever virus(ASFV),and use this method to detect the inactivation effect of various disinfectants on ASFV.We established a method called PMA-qPCR,which was used to evaluate the inactivation effect after treating ASFV with different kinds of disinfectants,and used it to explore the best treatment method of disinfectants.The results of PMA-qPCR showed that ASFV had different sensitivities to different types of disinfectants,with the highest sensitivity to potassium hydrogen persulfate complex solution,which could effectively inactivate ASFV within 20 min after diluting at 0.5%.The results had certain reference for the disinfection of biosecurity.