摘要
超高分辨率激光扫描共聚焦显微镜在生命科学研究领域中的应用日益广泛。该文以Zeiss LSM 880 Airyscan with STEDYCON超分辨率激光共聚焦显微镜为例,介绍了Airyscan技术和基于受激发射现象的超分辨技术(STED)的原理、应用和参数设置,以期能够为科研工作者究竟采用哪种模式进行图像采集提供参考,以获得最佳的成像效果。
Super-resolution laser scanning confocal microscope has been widely used in the field of life science research.Taking Zeiss LSM880 Airyscan with STEDYCON super resolution laser scanning confocal microscopy(LCSM)as an example,this paper introduces the principle,application and parameter setting of two super resolution technologies Airyscan and stimulated emission depletion(STED)based on stimulated emission phenomenon,in order to provide a reference for the scientific research workers on which mode to adopt for image acquisition to obtain the best imaging effect.
作者
路姣
孟国龙
余凌竹
LU Jiao;MENG Guolong;YU Lingzhu(National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610065,China)
出处
《实验科学与技术》
2023年第1期25-29,共5页
Experiment Science and Technology
基金
四川大学实验技术立项(SCU201195)。
