动物布鲁氏菌病不同初筛及确诊方法组合检测差异性比对分析

Different Primary Screenings for Animal Brucellosis and Comparative Analysis of Detection Differences in Diagnosis Method Combinations

为探讨布鲁氏菌病采用先初筛、后确诊不同方法及试剂组合检测结果的差异性,对实验室保存的133份牛、羊血清,以英国布病参考实验室RBT、SAT、APHA-cELISA方法为标准确定布病阳性血清73份、阴性血清60份。选择4种初筛方法共6种试剂、2种确诊方法共3种试剂,按照先初筛,对结果阳性样品再确诊的检测程序对确定的133份血清样品分别用6种方法(9种试剂)进行检测,结果表明:用18种组合方法检测牛阳性血清样品,符合率100%的有3种,检测结果假阴性率为0~53.33%;用12种组合方法检测羊阳性血清样品,符合率100%的有2种组合方法,检测结果假阴性率为0~50.00%。用所有方法检测牛阴性血清样品,符合率100%的有1种方法(试剂),检测结果假阳性率为0~77.78%;检测羊阴性血清样品,符合率100%的有4种方法(试剂),检测结果假阳性率为0~41.67%。应用不同的初筛、确诊组合方法检测同批样品,结果出现了多样性。建议布病初筛可优先选择iELISA、RBT、FPA,确诊可优先选择cELISA。

In order to explore brucellosis,the methods such aspreliminary screening at first,and then diagnosis confirmation,the differences of reagent combinationresults were used.The RBT,SAT.APHA-cELISA method of the British Brucellosis Laboratory were used as the standard,73 samples of brucellosis positive sera and 60 samples of negative sera were confirmed with the 133 samples of bo­vine and sheep serum stored in the laboratory.Four primary screening methods including 6 reagents,and 2 confirmatory methods including 3 reagents were selected,the preliminary screening was done first,and then the positive results were confirmed,the determined 133 serum samples were tested by 6 methods(9 reagents).The result showed that 18 kinds of combination methods were used to detect positive sera from cattle,3 of which had a 100%coincidence rate,and the false-negative rate was 0 to 53.33%;12 combinations were used to detect positive sera from sheep,and two combinations had a 100%coincidence rate,the false negative rate of test results was 0—50.00%.Cattle negative serum was tested by all methods,there was 1 method(reagent)with a compliance rate of 100%,and the false positive rate of test results was 0-77.78%jsheep negative serum was tested by all methods and the compliance rate was 100%by use of 4 methods(Reagent),the false positive rate of test results was 0—41.67%.In this study,different combination methods for preliminary screening and diagnosis were used to detect the same batch of samples,and the results showed diversity.It is recommended that iELISA,RBT,and FPA should be preferred for initial screening of brucellosis,and cELISA should be preferred for diagnosis.