miR-146b-5p和TRIM29在子宫内膜癌中的表达

Expression of miR-146b-5p and TRIM29 in endometrial carcinoma

目的探讨miRNA-146b-5p(miR-146b-5p)和三结构域蛋白29(TRIM29)在子宫内膜癌中的表达及临床意义。方法选取2019年10月—2020年10月徐州医科大学附属医院、徐州医科大学第二附属医院手术切除的子宫内膜癌组织60例、正常增生期子宫内膜组织30例。采用免疫组化法检测组织中TRIM29表达水平,qRT-PCR法检测miR-146b-5p、TRIM29 mRNA表达水平,Spearman法分析两者相关性,Kaplan-Meier法及Cox比例风险回归模型进行预后分析。结果免疫组化显示,TRIM29在子宫内膜癌组织中的阳性表达率(55.0%)低于正常增生期子宫内膜组织(76.7%)(P<0.05)。qRT-PCR检测显示,子宫内膜癌组织中miR-146b-5p的表达显著高于正常增生期子宫内膜组织,而TRIM29的表达明显低于正常增生期子宫内膜组织(P<0.05)。Spearman分析提示,miR-146b-5p与TRIM29的表达呈负相关(r=-0.595,P<0.05)。Kaplan-Meier分析显示,miR-146b-5p高表达组、TRIM29低表达组患者的预后较差(P<0.05)。Cox多因素回归分析显示,miR-146b-5p、TRIM29是影响子宫内膜癌患者预后的独立影响因素。结论子宫内膜癌组织中miR-146b-5p高表达,TRIM29低表达,二者的表达失调可能共同参与子宫内膜癌的发生发展。

Objective To investigate the expression of miRNA-146b-5p(miR-146b-5p)and tripartite motif protein 29(TRIM29)in endometrial cancer,and its clinical significance.Methods A total of 60 patients with endometrial cancer and 30 patients with normal proliferative endometrium were selected from the Affiliated Hospital of Xuzhou Medical University and the Second Affiliated Hospital of Xuzhou Medical University from October 2019 to October 2020.Their endometrial tissues were collected to detect the expression of TRIM29 by immunohistochemistry.The expression of miR-146b-5p and TRIM29 mRNA was measured by qRT-PCR.The correlation between the expression of miR-146b-5p and TRIM29 was evaluated by Spearman analysis.Then,Kaplan-Meier method and Cox proportional risk regression model were used to analyze the prognosis.Results According to immunohistochemical results,the positive expression rate of TRIM29 in endometrial carcinoma tissues(55.0%)was lower than that in normal proliferative endometrial tissues(76.7%)(P<0.05).Then,qRT-PCR results showed that the expression of miR-146b-5p in endometrial cancer tissues was significantly higher than that in normal proliferative endometrial tissues,while the expression of TRIM29 was significantly lower than that in normal proliferative endometrial tissues(P<0.05).Spearman analysis indicated that the expression of miR-146b-5p was negatively correlated with TRIM29(r=-0.595,P<0.05).Kaplan-Meier analysis showed that patients with high expression of miR-146b-5p and low expression of TRIM29 had poor prognosis(P<0.05).Cox multivariate regression analysis demonstrated that miR-146b-5p and TRIM29 were the independent factors affecting the prognosis of patients with endometrial cancer.Conclusions High expression of miR-146b-5p and low expression of TRIM29 in endometrial cancer tissues may be involved in the pathogenesis of endometrial cancer.