微波辅助快速合成卵清蛋白金纳米簇检测苦味酸

Microwave-assisted rapid synthesis of ovalbumin-stabilized gold nanoclusters for picric acid determination

本文采用微波加热法快速合成了卵清蛋白金纳米簇荧光探针(OVA-Au NCs)并应用于检测苦味酸(PA)。该探针在350 nm波长激发下,能发射红色荧光,最大发射荧光峰为680 nm,Stokes位移高达330 nm,可有效消除共振散射光的干扰。该合成方法与水热法相比,步骤简单快速,仅用50 s即可完成。由于PA的吸收光谱与OVA-Au NCs的激发光谱有较大范围的重叠,基于内滤效应(IFE) PA可选择性猝灭OVA-Au NCs的荧光,据此建立了检测苦味酸的新方法。在最佳实验条件下,在20~240μmol/L范围内,检测体系的荧光猝灭效率ΔF/F0与PA的浓度具有良好的线性关系,r为0.9985,检测限为6.4μmol/L。该方法简便、快速、准确,易于实现实时监测。

In this work, ovalbumin-stabilized gold nanoclusters(OVA-Au NCs) fluorescent nanoprobes were synthesized by microwave heating and applied to detect picric acid(PA). The nanoprobes emitted red fluorescence with the maximum fluorescence peak of 680 nm under the excitation wavelength of 350 nm, and the Stokes shifts could be up to 330 nm which could effectively eliminate the interference of resonance scattering light. Compared with hydrothermal method, the synthesis method was simple and fast, and only took 50 s. Due to the absorption peak of PA overlapped with the emission peak of OVA-Au NCs in a large range, PA could selectively quench the fluorescence of OVA-Au NCs based on the inner filter effect(IFE) and a quick response time(1 min). Therefore, a new and sensitive method for PA monitoring was established. Under the optimal conditions, the concentration of PA demonstrated a satisfactory linear correlation with the fluorescence quenching degree ?F/F0of the sensing system in the range of 20-240 μmol/L with the detection limit of 6.4 μmol/L. The proposed method is simple, fast, accurate, and easy to realize real-time monitoring.