表油菜素内酯对NaCl胁迫下马铃薯生长和块茎品质的影响

Effects of epibrassinolide on growth and tuber quality of potato under NaCl stress

为探究表油菜素内酯(EBR)对盐渍化土壤种植的马铃薯生长及块茎品质的影响,以‘尤金’、‘东农310’和‘东农312’3个马铃薯品种为试材,盆栽种植,在块茎形成期向土壤基质施加NaCl溶液,并对叶片喷施不同浓度(0.02和0.10mg/L)表油菜素内酯,以不施加NaCl和不喷施EBR植株为对照(CK),每隔14d测定叶片的SOD活性、MDA含量、PSII最大光化学量子产量(Fv/Fm)和叶绿素相对含量(SPAD),成熟期测定产量和块茎品质等指标。结果表明,在NaCl胁迫处理下3个品种马铃薯植株叶片SOD活性、SPAD和Fv/Fm均出现显著降低,MDA含量显著升高;而喷施EBR后各指标变化相反。NaCl胁迫处理后3个品种块茎中Fe含量较CK降低22.97%~46.43%、K含量较CK降低34.84%~41.67%;而喷施EBR可提高NaCl处理植株块茎Fe含量36.11%~163.66%、提高K含量30.41%~257.61%。NaCl胁迫处理后3个品种处理A的单株淀粉含量以及‘尤金’和‘东农310’块茎干物质含量和淀粉含量较CK均显著下降,喷施0.02mg/L EBR后3个品种单株淀粉含量较处理A提高19.03%~27.85%。综上,喷施浓度为0.02mg/L EBR可以显著降低土壤盐渍化对马铃薯生长和块茎品质的不利影响。

In order to explore the effects of epibrassinosteroid(EBR)on the growth and tuber quality of potato planted in saline soil,three potato varieties‘Youjin’,‘Dongnong 310’and‘Dongnong 312’were used as the research objects under potted planting condition.NaCl solution was applied to soil matrix at the tuber formation stage,and epibrassinolide with different concentrations(0.02and 0.10mg/L)was sprayed on the leaves,with no NaCl and no EBR treatment as CK.Then,leaf physiological indexes(SOD activity and MDA content),maximum photochemical efficiency of PSII(Fv/Fm)and chlorophyll relative content(SPAD)of leaves were measured every fifteen days.Yield and tuber quality of potato were measured at maturity stage.The results showed that:The SOD activity,SPAD,and Fv/Fmof the leaves of the three potato varieties were significantly decreased and the MDA content was significantly increased under NaCl stress.The changes of each index were opposite after spraying EBR.Under NaCl stress,Fe content in tubers of the three varieties decreased by 22.97%-46.43%and K content decreased by34.84%-41.67%compared with CK.Spraying EBR increased the Fe content in the tubers of plants under NaCl stress by 36.11%-163.66%and the K content by 30.41%-257.61%.Moreover,after NaCl stress treatment,the starch quality per plant,dry matter content and starch content of‘Youjin’and‘Dongnong 310’tubers of treatment A were significantly lower than those of CK,and the starch quality per plant of the three varieties with 0.02mg/L EBR was increased by 19.03%-27.85%compared with that of treatment A.In conclusion,spraying 0.02mg/L EBR could significantly reduce the adverse effects of soil salinization on the growth and tuber quality of potato.