类风湿性关节炎患者巨噬细胞关键基因及信号通路的生物信息学分析

Bioinformatics of key genes and signaling pathways in macrophages in patients with rheumatoid arthritis

目的基于生物信息学方法筛选类风湿性关节炎(RA)患者巨噬细胞关键基因及信号通路。方法下载基因表达数据集(GEO)数据库中RA患者滑膜巨噬细胞基因芯片,通过GEO2R功能获得差异表达基因,利用基因蛋白质相互作用关系检索工具(STRING)数据库构建蛋白质相互作用(PPI)网络获得关键基因,对关键基因进行基因本体论(GO)及京都基因和基因组学百科全书(KEGG)富集分析。结果通过整合3个基因芯片数据集,获得差异表达基因87个,进一步获得关键基因10个,富集分析发现关键基因与白细胞迁移、巨噬细胞分化、血小板脱颗粒、丝裂原活化蛋白激酶(MAPK)活性的正调控等生物学过程及磷脂酰肌醇3激酶/蛋白激酶B(PI3K/AKT)信号密切相关。结论RA患者巨噬细胞的关键基因与炎症反应机制相关,可能参与RA慢性炎症的发病机制。

Objective To screen key genes and signaling pathways in macrophages from patients with rheumatoid arthritis(RA)by bioinformatics.Methods Download the gene chip of synovial macrophages of RA patients from the Gene Expression Omnibus(GEO)database,obtain differentially expressed genes through the GEO2R function,and use the search tool for the retrival of interacting genes/proteins(STRING)database to construct a protein-protein interaction(PPI)network.Enrichment analysis was performed on key genes in Gene Ontology(GO)and Kyoto Encyclopedia of Gene and Genomics(KEGG).Results By integrating 3 gene chip datasets,87 differentially expressed genes were obtained,and 10 key genes were further obtained.The enrichment analysis found that key genes were associated with leukocyte migration,macrophage differentiation,platelet degranulation,mitogen-activated protein kinase(MAPK)activity.Other biological processes are closely related to phosphatidylinositol 3 kinase/protein kinase B(PI3K/AKT)signaling.Conclusion Key genes of macrophages in RA patients are associated with inflammatory response and may be involved in the pathogenesis of chronic inflammation in RA.