非小细胞肺癌中TLR4、Ki-67和Caspase-3的表达研究

The expression of TLR4,Ki-67 and Caspase-3 in non-small cell lung carcinoma

目的:探讨Toll样受体4(Toll-like receptor 4,TLR4)、核蛋白Ki-67和凋亡蛋白天冬氨酸蛋白水解酶3(cysteinyl aspartate specific proteinase 3,Caspase-3)在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)中的表达及意义。方法:用IHC(SP法)检测51例NSCLC与51例正常肺组织中TLR4、Ki-67及Caspase-3蛋白的表达情况,并分析其与临床病理特征之间的关系。结果:NSCLC组织中TLR4和Ki-67蛋白的表达均显著高于正常肺组织(均P<0.01);Caspase-3蛋白在NSCLC组织中的表达低于正常肺组织(P<0.05)。TLR4和Ki-67蛋白表达与分化程度有关(P<0.05),而与性别、年龄、肿块和组织学类型及淋巴结转移状态无关(P>0.05);Caspase-3蛋白表达与组织学类型和淋巴结转移状态有关(P<0.05),而与性别、年龄、肿块类型和分化程度无关(P>0.05)。NSCLC组织中TLR4及Ki-67表达呈正相关(P<0.01),而TLR4和Caspase-3、Ki-67和Caspase-3表达之间无相关性(P>0.05)。结论:NSCLC组织中TLR4和Ki-67蛋白表达增高及Caspase-3蛋白表达下调可能与NSCLC的发生、发展有密切的关系,TLR4和Ki-67指数高低与肺癌分化程度密切相关,对其的检测有助于判读肺癌的恶性程度及预测患者的预后情况。

Objective:To investigate the expression and significance of Toll-like receptor 4(TLR4),Ki-67 and cysteinyl aspartate specific proteinase 3(Caspase-3)in non-small cell lung carcinoma(NSCLC).Methods:Immunohistochemistry(SP)was used to evaluate the expression of TLR4,Ki-67 and Caspase-3 in 51 specimens of NSCLC and 51 samples of normal lung tissue,and their relationship with clinical-pathological features were analyzed.Results:The expression of TLR4 and Ki-67 protein in NSCLC tissues was significantly higher than that in adjacent normal lung samples(P<0.01),while the Caspase-3 protein was decreased(P<0.05).The expression of TLR4 and Ki-67 were correlated with the degree of differentiation(P<0.05),but had nothing to do with gender,age,the type of lump and histology,and lymph node metastasis status(P>0.05).Caspase-3 expression was related to the type of histology and the status of lymph node(P<0.05),but unrelated to gender,age,lump type and differentiation degree(P>0.05).TLR4 expression was positively correlated with Ki-67 in NSCLC tissues(P<0.01),while TLR4 and Ki-67expression were not correlated with Caspase-3(P>0.05).Conclusion:The up-regulation of TLR4 and Ki-67,and the downregulation of Caspase-3 in NSCLC might have close relationship with the occurrence and development of NSCLC,TLR4 and Ki-67 index were closely associated with lung cancer differentiation degree.Their detection contributed to interpretate the malignant degree and predict the prognosis of patients with lung carcinoma.