摘要
目的:探讨Toll样受体4(Toll-like receptor 4,TLR4)、核蛋白Ki-67和凋亡蛋白天冬氨酸蛋白水解酶3(cysteinyl aspartate specific proteinase 3,Caspase-3)在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)中的表达及意义。方法:用IHC(SP法)检测51例NSCLC与51例正常肺组织中TLR4、Ki-67及Caspase-3蛋白的表达情况,并分析其与临床病理特征之间的关系。结果:NSCLC组织中TLR4和Ki-67蛋白的表达均显著高于正常肺组织(均P<0.01);Caspase-3蛋白在NSCLC组织中的表达低于正常肺组织(P<0.05)。TLR4和Ki-67蛋白表达与分化程度有关(P<0.05),而与性别、年龄、肿块和组织学类型及淋巴结转移状态无关(P>0.05);Caspase-3蛋白表达与组织学类型和淋巴结转移状态有关(P<0.05),而与性别、年龄、肿块类型和分化程度无关(P>0.05)。NSCLC组织中TLR4及Ki-67表达呈正相关(P<0.01),而TLR4和Caspase-3、Ki-67和Caspase-3表达之间无相关性(P>0.05)。结论:NSCLC组织中TLR4和Ki-67蛋白表达增高及Caspase-3蛋白表达下调可能与NSCLC的发生、发展有密切的关系,TLR4和Ki-67指数高低与肺癌分化程度密切相关,对其的检测有助于判读肺癌的恶性程度及预测患者的预后情况。
Objective:To investigate the expression and significance of Toll-like receptor 4(TLR4),Ki-67 and cysteinyl aspartate specific proteinase 3(Caspase-3)in non-small cell lung carcinoma(NSCLC).Methods:Immunohistochemistry(SP)was used to evaluate the expression of TLR4,Ki-67 and Caspase-3 in 51 specimens of NSCLC and 51 samples of normal lung tissue,and their relationship with clinical-pathological features were analyzed.Results:The expression of TLR4 and Ki-67 protein in NSCLC tissues was significantly higher than that in adjacent normal lung samples(P<0.01),while the Caspase-3 protein was decreased(P<0.05).The expression of TLR4 and Ki-67 were correlated with the degree of differentiation(P<0.05),but had nothing to do with gender,age,the type of lump and histology,and lymph node metastasis status(P>0.05).Caspase-3 expression was related to the type of histology and the status of lymph node(P<0.05),but unrelated to gender,age,lump type and differentiation degree(P>0.05).TLR4 expression was positively correlated with Ki-67 in NSCLC tissues(P<0.01),while TLR4 and Ki-67expression were not correlated with Caspase-3(P>0.05).Conclusion:The up-regulation of TLR4 and Ki-67,and the downregulation of Caspase-3 in NSCLC might have close relationship with the occurrence and development of NSCLC,TLR4 and Ki-67 index were closely associated with lung cancer differentiation degree.Their detection contributed to interpretate the malignant degree and predict the prognosis of patients with lung carcinoma.
作者
龚长银
马建荣
陆海舟
GONG Changyin;MA Jianrong;LU Haizhou(Department of Pathology,Nantong Hospital of Traditional Chinese Medicine,Jiangsu Province,Nantong TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Nantong 226001)
出处
《南通大学学报(医学版)》
2022年第6期539-543,共5页
Journal of Nantong University(Medical sciences)