谷熟菌子实体2个发育时期的转录组比较分析

Comparative analysis of transcriptome of two developmental stages of fruit bodies of Lactarius deliciosus

以未开伞期和开伞期的谷熟菌子实体作为研究的材料,利用Illumina平台测序和生物信息学方法分析,探究谷熟菌子实体生长发育过程中不同基因表达的差异,预测对谷熟菌子实体生长和发育有影响的主要基因和代谢通路.结果显示谷熟菌未开伞期6.66 G和开伞期6.27 G的分析数据.KOG功能注释结果预测,谷熟菌子实体的基因成功注释在26基因功能类别中.GO富集预测结果谷熟菌在不同的生长阶段的生物过程、细胞成分和分子功能存在差异.基因表达水平与聚类分析结果显示,ATP酶和多功能伴侣家族基因是未开伞期和开伞期的主要高表达基因,在能量代谢和调控细胞周期过程中起着重要的作用.差异表达基因KEGG富集通路结果显示,影响谷熟菌2个生长发育过程中能量代谢的关键途径是氧化磷酸化途径.进一步研究结果显示,MAPK信号通路对谷熟菌子实体2个生长发育期起到关键作用,对于促进谷熟菌子实体未开伞期和开伞期有性生殖和调控菌丝体的极性生长过程中有着重要的影响.

Illumina sequencing and bioinformatics analysis were used to investigate the differences in gene expression during the growth and development of fruiting bodies of Lactarius deliciosus,and to predict the main genes and metabolic pathways that have influence on the growth and development of fruiting bodies of Lactarius deliciosus.The analysis results were as follows:6.66 G in the pre-umbrella period and 6.27 G in the umbrella period.The results of KOG functional annotation predicted that the successful gene annotation of the fruiting body of Lactarius deliciosus was in 26 gene functional categories.The results of GO enrichment predicted that there were differences in the biological processes,cell component processes and molecular functions of Lactarius deliciosus at different growth stages.The results of gene expression level and cluster analysis predicted that ATPase,the multifunctional chaperone family genes were the main overexpressed genes in the pre-umbrella period and in the umbrella period,which played an important role in energy metabolism and cell cycle regulation.The analysis of KEGG enrichment pathway of differentially expressed genes suggested that oxidative phosphorylation pathway was the key pathway affecting energy metabolism during the growth and development of Lactarius deliciosus.Further study results predicted that MAPK signaling pathway plays an important role in the growth and development of fruiting bodies of Lactarius deliciosus,and plays an important role in promoting sexual reproduction of fruiting bodies in the pre-umbrella period and the umbrella period stages and regulating the polar growth of mycelium.