不育患者精浆弹性蛋白酶、精子DNA碎片指数与精液常规参数的相关性研究

Correlation Between Seminal Plasma Elastase,Sperm DNA Fragment Index and Semen Routine Parameters in Infertile Patients

目的研究探讨不育患者精浆弹性蛋白酶(SPE)、精子DNA碎片指数(DFI)与精液常规参数的相关性。方法选取2020年06月~2022年06月就诊的155例男性不育患者为研究对象纳入观察组,另选同期进行健康体检的有正常生育史男性50例为对照组。采集观察组患者和对照组受试者精液样本,以酶联免疫吸附试验法检测SPE,以吖啶橙染色配合流式细胞仪技术检测DFI,按照《WHO人类精液检查与处理实验室手册》检测精液常规参数。对观察组、对照组SPE、DFI、精液常规参数进行统计比较,并采用Pearson检验对SPE、DFI与精液常规参数的相关性进行分析探讨。结果观察组患者的SPE、DFI高于对照组受试者(P<0.05)。观察组患者的精液常规参数包括精子活力、向前运动精子率、正常形态率均低于对照组受试者(P<0.05),两组精液量、精子浓度、精液pH值比较无差异性(P>0.05)。经Pearson检验分析,不育患者SPE、DFI与精子活力、向前运动精子率、正常形态率呈负相关性,与精液量、精子浓度、精液pH值无相关性。结论在不育男性患者中SPE、DFI呈现高表达,并且与精液常规参数精子活力、向前运动精子率、正常形态率呈负相关性。因此,通过对SPE、DFI的检测,可作为精液常规分析的补充指标,对于男性不育症的诊断及病情评估有重要意义。

Objective To study the correlation between seminal plasma elastase(SPE),sperm DNA fragment index(DFI)and semen routine parameters in infertile patients.Methods 155 male infertility patients admitted to Outpatients of the Affiliated Hospital of Youjiang Medical College for nationalities from June 2020 to June 2022 were selected as the study subjects and included in the observation group.50 male patients with normal reproductive history who underwent physical examination in our hospital Affiliated Hospital of Youjiang Medical College for Nationaliti were selected as the control group.Semen samples from patients in the observation group and subjects in the control group were collected,SPE was detected by enzyme-linked immunosorbent assay,DFI was detected by acridine orange staining and flow cytometry,and routine parameters of semen were detected according to WHO Manual of human semen examination and treatment laboratory.The SPE,DFI and semen routine parameters of the observation group and the control group were statistically compared,and the correlation between SPE,DFI and semen routine parameters was analyzed by Pearson test.Results The SPE and DFI in the observation group were higher than those in the control group(P<0.05).The routine parameters of semen in the observation group,including sperm motility,forward moving sperm rate and normal morphology rate,were lower than those in the control group(P<0.05).There was no difference in semen volume,sperm concentration and semen pH between the two groups(P>0.05).According to Pearson test,SPE and DFI in infertile patients were negatively correlated with sperm motility,forward moving sperm rate and normal morphology rate,but not with semen volume,sperm concentration and semen pH.Conclusions SPE and DFI are highly expressed in infertile male patients and negatively correlated with semen routine parameters such as sperm motility,forward moving sperm rate and normal morphology rate.Therefore,the detection of SPE and DFI can be used as a supplementary index for semen routine analysis,which is of great significance for the diagnosis and evaluation of male infertility.