高压氧处理对妊娠期糖尿病小鼠胰岛素敏感性以及血管内皮功能的影响

Effects of hyperbaric oxygen on insulin sensitivity and endothelial function in gestational diabetic mice

目的:探讨高压氧处理对妊娠期糖尿病小鼠胰岛素敏感性及内皮功能的影响。方法:选取60只清洁级C57BL/6J小鼠,构建妊娠期糖尿病模型。按照数字表法随机分为模型组、阳性组、高压氧组,每组20只。模型组为单纯糖尿病小鼠,阳性组为糖尿病小鼠胰岛素抵抗,高压氧组为糖尿病小鼠胰岛素抵抗后采用高压氧处理。采用酶联免疫吸附实验测定小鼠空腹血浆胰岛素水平,计算胰岛素抵抗指数(HOMA-IR)。高效液相色谱法测定乙酰胆碱介导的血管内皮依赖性舒张反应和硝酸甘油介导的非内皮依赖性舒张反应,以此来评估小鼠血管内皮功能。结果:与模型组比较,阳性组空腹血糖、空腹胰岛素水平明显降低,肝糖原水平明显升高(P<0.05);与阳性组比较,高压氧组空腹血糖、空腹胰岛素水平明显降低,肝糖原水平明显升高(P<0.05)。与模型组比较,阳性组神经肽Y表达量、HOMA-IR明显降低(P<0.05);与阳性组比较,高压氧组神经肽Y表达量、HOMA-IR明显降低(P<0.05)。与模型组比较,阳性组乙酰胆碱、硝酸甘油水平明显升高(P<0.05);与阳性组比较,高压氧组乙酰胆碱、硝酸甘油水平明显升高(P<0.05)。结论:高压氧处理可改善妊娠期糖尿病小鼠空腹血糖和神经肽Y水平,提高胰岛素敏感性,同时还能改善血管内皮功能。

Objective:To explore and analyze the effect of hyperbaric oxygen(HBO)on insulin sensitivity and endothelial function in gestational diabetic mice.Methods:A total of 60 clean-class pregnant C57BL/6J mice were selected to establish gestational diabetic mouse models,and they were randomly divided into model group,positive group,and HBO group according to the digital table method,with 20 mice in each group.The model group consisted of diabetic mice,and the positive group consisted of diabetic mice with insulin resistance.The diabetic mice with insulin resistance in the HBO group was treated with HBO.The fasting insulin level was measured by the enzyme-linked immunosorbent assay(ELISA)and the homeostatic model assessment of insulin resistance(HOMA-IR)was calculated.The endothelium-dependent dilation mediated by acetylcholine(ACh)and the endothelium-independent dilation mediated by nitroglycerin(NTG)were measured by high performance liquid chromatography(HPLC)to evaluate the endothelial function of mice.Results:Compared with the model group,the positive group had significantly decreased fasting blood glucose and fasting insulin levels,and significantly increased hepatic glycogen level(P<0.05).Compared with the positive group,the HBO group had significantly decreased fasting blood glucose and fasting insulin levels,and significantly increased hepatic glycogen level(P<0.05).Compared with the model group,the positive group had higher expression of neuropeptide Y and lower HOMA-IR(P<0.05);compared with the positive group,the HBO group had significantly higher expression of neuropeptide Y,and significantly lower HOMA-IR(P<0.05).Compared with the model group,the levels of ACh and NTG in the positive group were significantly increased(P<0.05);while compared with the positive group,the levels of ACh and NTG in the HBO group were significantly higher(P<0.05).Conclusion:HBO can improve the levels of fasting blood glucose and neuropeptide Y,enhance insulin sensitivity,and improve vascular endothelial function in mice with gestational diabetes mellitus.