摘要
The combination of optogenetics and electrophysiological recording enables high-precision bidirectional interactions between neural interfaces and neural circuits,which provides a promising approach for the study of progressive neurophysiological phenomena.Opto-electrophysiological neural probes with sufficient flexibility and biocompatibility are desirable to match the low mechanical stiffness of brain tissue for chronic reliable performance.However,lack of rigidity poses challenges for the accurate implantation of flexible neural probes with less invasiveness.Herein,we report a hybrid probe(Silk-Optrode)consisting of a silk protein optical fiber and multiple flexible microelectrode arrays.The Silk-Optrode can be accurately inserted into the brain and perform synchronized optogenetic stimulation and multichannel recording in freely behaving animals.Silk plays an important role due to its high transparency,excellent biocompatibility,and mechanical controllability.Through the hydration of the silk optical fiber,the Silk-Optrode probe enables itself to actively adapt to the environment after implantation and reduce its own mechanical stiffness to implant into the brain with high fidelity while maintaining mechanical compliance with the surrounding tissue.The probes with 128 recording channels can detect high-yield well-isolated single units while performing intracranial light stimulation with low optical losses,surpassing previous work of a similar type.Two months of post-surgery results suggested that as-reported Silk-Optrode probes exhibit better implant-neural interfaces with less immunoreactive glial responses and tissue lesions.
基金
partially supported by the National Key R&D Program of China(Grant Nos.2019YFA0905200,2021ZD0201600,2021YFC2501500,2021YFF1200700,2022ZD0209300,2022ZD0212300)
National Natural Science Foundation of China(Grant No.61974154)
Key Research Program of Frontier Sciences,CAS(Grant No.ZDBSLY-JSC024)
Shanghai Pilot Program for Basic Research—Chinese Academy of Science,Shanghai Branch(Grant No.JCYJ-SHFY-2022-01)
Shanghai Municipal Science and Technology Major Project(Grant No.2021SHZDZX)
CAS Pioneer Hundred Talents Program,Shanghai Pujiang Program(Grant Nos.19PJ1410900,21PJ1415100)
the Science and Technology Commission Foundation of Shanghai(No.21JM0010200)
Shanghai Rising-Star Program(Grant No.22QA1410900)
the Innovative Research Team of High-level Local Universities in Shanghai,the Jiangxi Province 03 Special Project and 5G Project(Grant No.20212ABC03W07)
Fund for Central Government in Guidance of Local Science and Technology Development(Grant No.20201ZDE04013)
Special Fund for Science and Technology Innovation Strategy of Guangdong Province(Grant Nos.2021B0909060002,2021B0909050004)。