广西人群microRNA-208基因多态性分布特征及与血脂的关系

Distribution of microRNA-208 polymorphism and its correlation with blood lipids in Guangxi people

目的探究microRNA-208(miR-208)基因rs10136106位点在广西人群中的分布特征,并将其多态性与其他地区人群比较,进一步探究其多态性与血脂水平的关系。方法选取2021年1月—2021年6月来桂林医学院附属医院体检的无亲缘关系的健康群众168例。采用高通量SNPscan技术检测研究对象rs10136106位点的基因分型;对比研究对象的血脂指标,并分析其差异。结果基因分型结果显示rs10136106存在AG(3.57%)和GG(96.43%)2种基因型,等位基因A、G频率分别为1.79%、98.21%。男女性间rs10136106位点的基因型频率、等位基因频率比较,差异无统计学意义(P>0.05)。广西人群分别与美国犹他州人群、尼日利亚伊巴丹约鲁人群及意大利托斯卡纳人群rs10136106位点基因型频率、等位基因频率比较,差异有统计学意义(P<0.05),而广西人群分别与日本东京人群、中国北京汉族人群基因型频率、等位基因频率比较,差异无统计学意义(P>0.05)。广西人群rs10136106位点AG和GG基因型携带者甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇比较,差异无统计学意义(P>0.05),而AG基因型携带者总胆固醇较GG基因型携带者低(P<0.05)。结论广西人群miR-208基因rs10136106位点单核苷酸多态性与其他地区人群存在不同程度的差异,rs10136106位点多态性与总胆固醇相关。

Objective To explore the distribution of microRNA-208 rs10136106 polymorphism in Guangxi people and to compare the distribution with that of people in other regions,and further to investigate the correlation between rs10136106 polymorphism and the levels of blood lipids.Methods A total of 168 unrelated healthy individuals undergoing health checkup in Affiliated Hospital of Guilin Medical University from January 2021 to June 2021 were enrolled.The high-throughput SNPscan technology was used to determine the genotyping of rs10136106.The blood lipid profiles in all individuals were collected,and the differences among them were analyzed.Results Two genotypes,AG(3.57%)and GG(96.43%),were found at the rs10136106 locus,and the frequencies of A and G alleles were 1.79%and 98.21%.There was no significant difference in the genotype frequency and allele frequency at the rs10136106 locus between males and females among the Guangxi people(P>0.05).The genotype frequency and allele frequency at the rs10136106 locus in the Guangxi people differed from those in the Utah people,Yoruba people,and Tuscany people(P<0.05),while they were not different from those in the Tokyo people and Han people from Beijing(P>0.05).There was no difference in the levels of triglyceride,high density lipoprotein cholesterol or low density lipoprotein cholesterol between AG and GG carriers(P>0.05),whereas the level of total cholesterol in AG carriers was lower than that in GG carriers(P<0.05).Conclusions There are different degrees of variation in the microRNA-208 rs10136106 polymorphism among Guangxi people and those from other regions.Besides,the rs10136106 polymorphism is related to the level of total cholesterol.