Nesfatin-1、瘦素在肥胖儿童的表达水平及临床意义

Expression levels and clinical significance of Nesfatin-1 and leptin in children with obesity

目的检测Nesfatin-1和瘦素在肥胖儿童外周血中的表达水平,探究二者表达水平的相关性和临床意义。方法选取2022年1-4月该院门诊或住院的80例患儿为研究对象,根据BMI将40例肥胖儿童作为肥胖组,40例BMI正常的健康儿童作为对照组。采集两组外周血,采用贝克曼全自动生化分析仪(AU5800)检测血清生化指标水平,采用罗氏化学发光分析仪(cobas 602)检测血清中胰岛素水平,采用ELISA检测血清Nesfatin-1和瘦素水平。结果与对照组比较,肥胖组Nesfatin-1[(6599.84±3647.73)ng/L vs.(1022.04±422.99)ng/L]、瘦素水平[(8530.85±4603.02)ng/L vs.(1695.01±1153.63)ng/L]更高,差异有统计学意义(P<0.05)。两组Nesfatin-1、瘦素水平与BMI呈正相关,Nesfatin-1和瘦素水平也呈正相关(P<0.05)。结论Nesfatin-1和瘦素协同参与肥胖儿童体重调节。

Objective To detect the expression levels of Nesfatin-1 and leptin in the peripheral blood of obese children,and further to explore the correlation and the clinical significance of the expression level of Nesfatin-1 and leptin.Methods From January to April,2022,a total of 80 outpatients or inpatients in this hospital were selected as research objects.According to body mass index(BMI),40 obese children were taken as the obesity group and 40 healthy children with BMI in the normal range were selected as the control group.The peripheral blood of the two groups was collected,and the serum biochemical indexes were detected by Beckman fully automatic biochemical analyzer(AU5800),insulin level was detected by Roche chemiluminescent analyzer(cobas 602),and serum Nesfatin-1 and leptin levels were detected by ELISA.Results Compared with the control group,the levels of Nesfatin-1[(6599.84±3647.73)ng/L vs.(1022.04±422.99)ng/L]and leptin[(8530.85±4603.02)ng/L vs.(1695.01±1153.63)ng/L]in the obesity group were higher,and the differences were all significantly significant(P<0.05).The levels of Nesfatin-1 and leptin in the two groups were positively correlated with BMI,and the levels of Nesfatin-1 and leptin were also positively correlated(P<0.05).Conclusion Nesfatin-1 and leptin are involved in the weight regulation of obese children.