应用大鼠股血管进行侧-侧吻合训练的可行性研究

Feasibility study on the training of side-to-side microvascular anastomosis using the femoral vessels in rats

目的探讨应用大鼠股动、静脉进行侧-侧血管吻合训练的可行性。方法实验采用20只雄性SD大鼠,分离左侧股动脉、股静脉,采用连续缝合的方式进行侧-侧吻合。测量大鼠股动脉和股静脉的直径、吻合口的长度、吻合口与临时阻断夹的距离,同时记录缝合时间,并采用Acland试验对吻合手术后即刻、术后30 min及术后7 d的吻合口通畅性进行评价。结果20只大鼠均顺利完成股动脉-股静脉侧-侧吻合(共20侧),手术成功率为100%。大鼠股动脉、股静脉的直径分别为(0.68±0.11)mm(0.50~0.80 mm)、(1.21±0.18)mm(1.00~1.50 mm),吻合口的长度为(2.32±0.22)mm(2.00~2.60 mm),吻合口与临时阻断夹的距离为1.00~2.50 mm,总吻合时间为(29.2±3.0)min(26~34 min)。术后即刻、术后30 min及术后7 d的吻合口通畅率均为100%。结论大鼠股动脉-股静脉侧-侧吻合是一个有效、可行的侧-侧血管吻合训练方法。

Objective To investigate the feasibility of establishing a side-to-side microvascular anastomosis training model by using the femoral artery and vein in rats.Methods Twenty male SD rats were used in the study.After left-sided femoral arteries and veins were carefully dissected,side-to-side microvascular anastomosis was performed between femoral artery and vein via continuous suturing technique.The diameter of femora artery and vein,the length of the arteriotomy or venotomy,the distance between the corner of the arteriotomy and the temporary clip and suturing time were documented.Patency of those anastomoses was evaluated immediately,30 min and 7 days after the procedure.Results Twenty side-to-side microvascular anastomoses between femoral artery and vein were successfully performed in 20 rats,and the operation success rate was 100%.The diameter of femoral artery and vein was 0.68±0.11 mm(0.50-0.80 mm)and 1.21±0.18 mm(1.00-1.50 mm)respectively.The length of the arteriotomy or venotomy was 2.32±0.22 mm(2.00-2.60 mm).The distance between the corner of the arteriotomy and the temporary clip was 1.00-2.50 mm.The total suturing time was 29.2±3.0 min(26-34 min).The patency rate of 100%was achieved immediately,30 min and 7 days after the procedure.Conclusion Side-to-side microvascular anastomosis training model between rat femoral artery and vein is a feasible and effective side-to-side microvascular anastomosis training model.