磷源调控蛋白核小球藻胞外聚合物及生物絮凝性

Phosphorus source regulates extracellular polymers and bioflocculation of Chlorella pyrenoidosa

产油微藻的高效回收是限制利用微藻生产生物柴油的瓶颈问题,其中生物促沉降成为研究的热点,然而通过磷源调控微藻的生长、产油和生物絮凝等特性的研究尚较稀少.选择4组(无机磷酸盐(IP)、环核苷单磷酸盐(cNMP)、非环核苷单磷酸盐(NMP)和其他有机磷酸盐(OP))共59种不同的磷源,用以培养蛋白核小球藻(Chlorella pyrenoidosa),并对其生长、产油、细胞沉降及胞外聚合物的组分进行研究,发现IP组对Chlorella pyrenoidosa的生长促进作用最高(藻密度平均达2.92×10^(8)cells/mL);OP组培养的藻细胞含油率达到了17.45%,高于其他3组;而cNMP组的平均沉降效果最好,可达13.79mm/min.综合来看,亚甲基二磷酸、磷酸三乙酯和6-磷酸葡萄糖是用以Chlorella pyrenoidosa产油培养综合效益最好的3种磷源.

The efficient harvesting of oil-producing microalgae is a bottleneck problem that restricts the use of microalgae to produce biodiesel.Among them,the biological method has become a research hotspot.However,research on how phosphorus affects the growth,oil production,and bioflocculation of microalgae are still limited.In this study,four groups(IP,OP,NMP,and cNMP)of 59kinds of phosphorus were selected to cultivate Chlorella pyrenoidosa,and their growth,oil production,cell settling,and composition of extracellular polymers were studied.It was found that the IP group had the highest growth promotion effect on Chlorella pyrenoidosa(average algal cell density was 2.92×10^(8) cells/mL),and the oil levels of algal cells cultured in the OP group reached 17.45%,higher than the other three groups,while the average settling velocity of cNMP group was the best,reaching 13.79mm/min.In conclusion,methylene piphosphonic acid,triethyl phosphate,and 6-phospho-gluamic acid are the phosphorus with the best comprehensive benefits for cultivating Chlorella pyrenoidosa for oil production.