斯钙素2的表达与宫颈鳞状细胞癌的发生及预后不良相关

Expression of STC2 is associated with the occurrence and poor prognosis of cervical squamous cell carcinoma

目的:探讨斯钙素2(STC2)在宫颈鳞状细胞癌(SCC)的表达,并分析宫颈SCC组织中STC2与斯钙素1(STC1)表达的相关性,分析其与临床病理特征的关系,宫颈SCC化疗前后表达的变化,以及其表达与预后的关系。方法:采用免疫组化法(Envision二步法)检测82例宫颈SCC(其中包括42例化疗处理前后对照,40例未进行任何治疗的根治性手术切除病例)、30例高度上皮内病变(HSIL)、30例低度上皮内病变(LSIL)、20例慢性宫颈炎组织中STC2蛋白的表达水平,检测宫颈82例SCC(其中包括42例化疗处理前宫颈活检SCC,40例未进行任何治疗的根治性手术切除病例)组织中STC1和STC2的表达,分析STC2的表达与临床病理特征的关系及STC1与STC2表达的相关性。结果:STC2蛋白在宫颈SCC、HSIL、LSIL、慢性宫颈炎阳性表达率依次降低,阳性率分别为89.02%(73/82)、86.67%(26/30)、63.33%(19/30)、15.00%(3/20),宫颈SCC与HSIL表达水平比较,差异无统计学意义(P>0.05),其余指标比较,差异均有统计学意义(P<0.05)。宫颈SCC组织中STC2阳性率高于STC1(P<0.05),且两者的表达正相关(r=0.359,P<0.05)。STC2的表达与淋巴结转移相关(P<0.05);与患者年龄、分化程度、FIGO分期、癌组织浸润深度均无关(P>0.05)。同一宫颈SCC患病群体化疗后STC2的表达降低(P<0.05),STC2在有化疗反应的宫颈SCC组织中的表达均低于无化疗反应(P<0.05)。STC2表达水平与生存率负相关(P<0.05)。结论:STC2在宫颈SCC组织中高表达,其表达与淋巴结转移、化疗耐受以及预后相关,有望成为宫颈SCC新的治疗靶点。

Objective:To investigate the expression of stanniocalcin 2(STC2) in cervical squamous cell carcinoma(SCC) and analyze the correlation between stanniocalcin 1(STC1) and STC2 in cervical SCC.The relationship between STC2 and clinicopathological features, the change of STC2 expression before and after cervical SCC chemotherapy, and the relationship between STC2 expression and prognosis were analyzed.Methods:Immunohistochemical method(Envision two-step method) was used to detect the expression levels of STC2 in 82 cases of cervical SCC(including 42 cases of cervical SCC before and after chemotherapy, and 40 cases of radical surgical resection without any treatment),30 cases of high grade squamous intraepithelial lesion(HSIL),30 cases of low grade squamous intraepithelial lesion(LSIL) and 20 cases of chronic cervicitis.The expression of STC1 and STC2 was detected in 82 cases of cervical SCC(including 42 cases of cervical biopsy SCC before chemotherapy, and 40 cases of radical surgical resection without any treatment),and the relationship between STC2 expression and clinicopathological features as well as the correlation between STC1 and STC2 expression was analyzed.Results:The positive expression rates of STC2 protein in cervical SCC,HSIL,LSIL and chronic cervicitis decreased successively, and the positive rates were 89.02%(73/82),86.67%(26/30),63.33%(19/30) and 15.00%(3/20),respectively.There was no significant difference in the expression of SCC and HSIL(P>0.05),and the other differences were statistically significant(P<0.05).The positive rate of STC2 in cervical SCC tissue was higher than that of STC1(P<0.05),and the expression of STC2 and STC1 was positively correlated(r=0.359,P<0.05).The expression of STC2 was correlated with lymph node metastasis(P<0.05).There was no significant correlation with age, degree of differentiation, FIGO stage and depth of tumor tissue invasion(P>0.05).The expression of STC2 in the same cervical SCC patients decreased after chemotherapy(P<0.05),and the expression of STC2 in the cervical SCC tissues with chemotherapy response was lower than that without chemotherapy response(P<0.05).The expression of STC2 was negatively correlated with survival(P<0.05).Conclusion:STC2 is highly expressed in cervical SCC tissues, and its expression is correlated with lymph node metastasis, chemotherapy tolerance and prognosis, which is expected to become a new therapeutic target for cervical SCC.