紫草素对完全弗氏佐剂诱导的类风湿性关节炎小鼠模型的治疗作用

Therapeutic effects of shikonin on complete Freund’s adjuvant-induced rheumatoid arthritis mouse model

为探讨紫草素对类风湿关节炎(RA)的影响,将ICR小鼠随机分为4组:正常对照组和3个试验组(模型组、阳性对照组、紫草素组)。造模15 d后给药治疗60 d,阳性对照组灌胃美洛昔康[50 mg/(kg·d)],紫草素组灌胃紫草素[2 mg/(kg·d)],正常对照组和模型组灌胃等体积的生理盐水,分离小鼠右足踝关节部位,对滑膜炎症程度(0-3级)和软骨破坏程度(0-4级)以及骨浸润进行评估,分离小鼠胸腺和脾脏,计算胸腺指数和脾脏指数,并对各组小鼠进行组织病理学的评价和部分血清生化指标(致炎因子和抗氧化因子)的分析测定。结果表明,紫草素能显著改善小鼠的关节红肿症状。紫草素组中一氧化氮(NO)、肿瘤因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)含量显著低于AA模型组,血清中丙二醛(MDA)含量显著低于AA模型组,超氧化物歧化酶(SOD)酶活力显著高于AA模型组,差异有统计学意义(P<0.05或P<0.01)。紫草素可以通过降低炎症因子水平,提高机体抗氧化能力从而减轻RA的炎症反应。

To investigate the effect of shikonin(SHI)on rheumatoid arthritis(RA),ICR mice were randomly divided into 4 groups,including the normal group and three experimental groups(model group,positive drug group and SHI group).After 15 days of modeling,the drug treatment losted for 60 days.The positive drug group was given meloxicam 50 mg/(kg·d),the SHI group was given shikonin 2 mg/(kg·d),and the normal group and model group were given normal saline.The right ankle joint of the mice was separated,and the degree of synovial inflammation(grade 0-3),cartilage destruction(grade 0-4)and bone infiltration were evaluated.The thymus and spleen of mice were separated and thymus index and spleen index were calculated.Histopathological evaluation and some serum biochemical indexes(including inflammatory factors and antioxidant factors)were analyzed and determined.The results showed that shikonin could significantly improve the symptoms of joint redness in mice.The levels of nitric oxide(NO),tumor factorα(TNF-α),interleukin 1β(IL-1β)and interleukin 6(IL-6),in the SHI group were significantly lower than those in the AA model group,the level of malondialdehyde(MDA)in serum as significantly lower than that in the AA model group,and the enzyme activity of superoxide dismutase(SOD)was significantly higher than that in the AA model group.The difference was statistically significaht(P<0.05 or P<0.01).Shikonin could relieve the inflammatory response of RA by reducing the level of inflammatory factors and improving the antioxidant capacity of the body.