百色市及周边地区人乳头状瘤病毒感染情况分析研究

Analysis of HPV infection in Baise city and its surrounding areas

目的分析百色市及周边地区女性宫颈上皮细胞感染人乳头状瘤病毒(human papillomavirus,HPV)的状况与基因型别分布情况,为该地区妇女预防HPV感染和宫颈癌筛查提供流行病学依据。方法收集2019年1月至2021年6月百色市及周边地区进行宫颈标本HPV分型检查的研究对象,共计24680例,采用PCR-反向点杂交法(PCR-RBD)检测18种高危HPV(high-risk HPV,HR-HPV)和5种低危HPV基因型的分布情况,分析HPV感染率、感染型别与患者不同年龄、不同宫颈病变间的关系,也分析HR-HPV单一和多重感染在不同宫颈病变组间是否存在差异及HPV16/18感染率与宫颈病变程度的关联性。结果研究对象中,HPV感染阳性率为26.92%(6644/24680),主要高危型包括HPV52、HPV16、HPV51、HPV58、HPV53和HPV68等6型,这些HR-HPV的感染率在≤21岁和≥61岁组中较高,且HR-HPV(尤其是HPV16/18)感染率随宫颈病变严重程度的增加而增高,这种差异具有统计学意义(P<0.05),但HR-HPV单一和多重感染阳性率在不同程度宫颈病变组中的差异无统计学意义(P>0.05)。结论百色市及周边地区HR-HPV感染型别与我国常见型别有差异,在≤21岁和≥61岁的妇女中感染率高,且HR-HPV(尤其是HPV16/18)感染率随宫颈病变严重程度的增加而增高。提示百色市及周边地区女性尤其是具有HPV16/18感染者要注意宫颈病变的筛查。

Objective To analyze the status and genotype distribution of human papillomavirus(HPV)infection in the cervical epithelial cells of women in Baise city and its surrounding areas,so as to provide epidemiologic data for preventing HPV infection and screening cervical cancer among women from this area.Methods A total of 24680 subjects were collected for cervical HPV typing in Baise city and its surrounding areas from January 2019 to June 2021.PCR-reverse dot hybridization(PCR-RBD)was adopted to detect the distribution of 18 high-risk HPV(HR-HPV)genotypes and 5 low-risk HPV types.This research analyzed the relationship between HPV infection rate,infection type and different ages and different cervical lesions.It also analyzed the difference of single and multiple HR-HPV infection among groups with different cervical lesions and the correlation between HPV16/18 infection rate and the degree of cervical lesions.Results The positive rate of HPV infection among all study subjects was 26.92%(6644/24680),and the main high-risk types included HPV52,HPV16,HPV51,HPV58,HPV53 and HPV68.These HR-HPV infection rates were higher in the group≤21 years old and the group≥61 years old.Furthermore,the infection rate of HR-HPV(especially HPV16/18)increased with the increase of the severity of cervical lesions,and the difference was statistically significant(P<0.05).However,there was no significant difference in the positive rate of single and multiple HR-HPV infection among groups with different degrees of cervical lesions(P>0.05).Conclusion The types of HR-HPV infection in Baise city and its surrounding areas are different from the common types in our country.The infection rate is high in women≤21 years old and those≥61 years old;the infection rate of HR-HPV(especially HPV16/18)increases with the increase of the severity of cervical lesions.It is suggested that women in Baise city and its surrounding areas,especially those infected with HPV16/18,should attach importance to cervical screening.