莱菔硫烷对RhoC敲低的口腔鳞癌细胞的调控作用

Effect of sulforaphane on RhoC/shRNA knocking down oral squamous cell carcinoma cells

目的 观察莱菔硫烷(sulforaphane,SFN)对RhoC稳定敲低的口腔鳞癌细胞系CAL27 RhoC/shRNA的影响。方法 选取口腔鳞癌细胞系CAL27 RhoC/shRNA进行培养,用不同浓度的SFN处理对数期CAL27RhoC/shRNA细胞,通过RT-PCR和western blot检测SFN对细胞中Nrf2 RNA和蛋白水平的影响。分别通过MTT实验、克隆形成实验和侵袭实验检测SFN对口腔鳞癌细胞增殖能力、克隆形成能力和侵袭能力的影响;PHOD染色检测SFN对口腔鳞癌细胞F-actin聚合能力的影响。结果 应用SFN能有效抑制口腔鳞癌细胞CAL27 RhoC/shRNA的增殖能力、克隆形成能力、侵袭能力和F-actin蛋白聚合能力。结论 SFN可减弱RhoC敲低的口腔鳞癌细胞的恶性能力。

Objective To investigate the effect of sulforaphane(SFN) on RhoC/shRNA knocking down oral squamous cell carcinoma(OSCC) cells. Methods OSCC cell line CAL27 RhoC/shRNA was cultured in DMEM high glucose medium with 10% FBS and the logarithmic phase CAL27 RhoC/shRNA cells were treated with different concentrations of SFN. The effect of SFN on Nrf2 gene in RNA and protein level was detected by RT-PCR and western blot assay, respectively.The effect of SFN on the proliferation, colony formation and invasion ability of OSCC was examined by MTT assay, colony formation experiment and transwell assay. The effect of SFN on the F-actin polymerization ability of OSCC was detected by PHOD staining assay. Results SFN could effectively inhibit the proliferation, colony formation, invasion and F-actin protein polymerization ability of OSCC cells CAL27 RhoC/shRNA. Conclusions SFN could attenuate the malignant ability of RhoC/shRNA knocking down oral squamous cell carcinoma cells.