氟苯尼考胁迫对土壤解磷菌解磷能力的影响

Effect of Florfenicol Stress on Phosphate-solubilizing Ability of Soil Phosphate-solubilizing Bacteria

从土壤中分离得到10株解磷菌,将菌株接种至LB液体培养基中,30℃培养24 h作为种子液。在无机磷液体培养基中加入氟苯尼考,使培养基含药浓度分别为0、0.1、1.0、10.0、50.0μg/mL。按照1%的接种量接种种子液,30℃培养5 d,每24 h取2 mL培养液,利用钼锑抗分光光度法测定培养液中速效磷含量。结果表明,10株解磷菌在50.0μg/mL药物浓度下,培养基中速效磷含量明显低于其他药物浓度组;大部分菌株在药物浓度0~50.0μg/mL菌液中细菌数量变化不明显。说明氟苯尼考对解磷菌的解磷能力产生了抑制作用,但与细菌的生长繁殖并无明显相关性。

10 phosphate-solubilizing bacteria were isolated from soil,inoculated into LB liquid medium,and cultured at 30°C for 24 h as seed solution.Florfenicol was added to the inorganic phosphorus liquid medium,and the drug concentrations in the medium were 0,0.1,1.0,10.0 and 50.0μg/mL,respectively.Seed solution was inoculated with 1%inoculum,cultured at 30°C for 5 d,and 2 mL culture medium was taken every 24 h.The content of available phosphorus in culture medium was determined by molybdenum antimony anti-spectrophotometry.The results showed that the content of available phosphorus in the medium of 10 phosphate-solubilizing bacteria at 50.0μg/mL was significantly lower than that of other drug concentrations.Most of the strains in the bacterial solution with drug concentration range of 0-50.0μg/mL,the number of bacteria did not change significantly.This indicated that florfenicol inhibited the phosphate-solubilizing ability of phosphate-solubilizing bacteria,but had no significant correlation with the growth and reproduction of bacteria.