鸡传染性支气管炎·H9亚型禽流感病毒与鸡毒支原体混合感染的诊治

Diagnosis and Treatment of Mixed Infection of Avian Infectious Bronchitis Virus,Avian Influenza H9 Subtype Virus,Mycoplasma gallisepticum

通过病理学解剖、细菌分离与培养、病毒分离鉴定、动物回归试验和分子生物学检测,对山东省日照市某发病鸡场5只病死鸡进行诊断。病理学剖检显示,病鸡支气管栓塞,气管喉头出血,肺出血水肿、颜色发黑,输尿管有尿酸盐沉积,气囊增厚、有干酪物渗出;从发病鸡组织中未分离出细菌;分子生物学检测结果发现,鸡传染性支气管炎病毒、鸡毒支原体和H9亚型禽流感病毒检测结果均呈阳性。经初步推断,发病鸡群存在鸡毒支原体隐性感染,又继发感染鸡传染性支气管炎和H9亚型禽流感病毒,多种病原混合感染造成鸡只死亡。建议该鸡场加强鸡传染性支气管炎和H9亚型禽流感防控措施,投喂抗病毒类药物。

Through the methods of pathological anatomy,bacterial isolation and culture,virus isolation and identification,animal regression test and molecular biological detection,the etiology of 5 dead chickens in a chicken farm in Rizhao City,Shandong Province was diagnosed.Pathological autopsy showed bronchial embolism,tracheo laryngeal hemorrhage,pulmonary hemorrhage,edema,dark color,urate deposition in ureter,thickening of air bag and exudation of cheese;no bacteria were isolated from the diseased chicken tissues;molecular biological tests showed that the avian infectious bronchitis virus,Mycoplasma gallisepticum and avian influenza H9 subtype were positive.It was preliminarily inferred that the cause of the chicken herd was the secondary infection of chicken infectious bronchitis and avian influenza H9 subtype on the basis of the invisible infection of Mycoplasma gallisepticum,and the casualties caused by the mixed infection of multiple pathogens.It is suggested that the chicken farm strengthen the immune prevention and control measures of chicken infectious bronchitis and avian influenza H9 subtype,and use antiviral drugs.