不同FSH处理方法对多浪羊FSH、LH、E2和P4激素水平及发情的影响

Effects of Different FSH Treatment Methods on FSH, LH, E2 and P4 Hormone Levels and Estrus in Duolang Sheep

[目的]为了研究FSH不同注射方法对多浪羊繁殖性能的影响。[方法]试验将172只多浪羊母羊随机分为A、B、C 3个试验组和1个对照组(D)。在相同饲养条件下,于阴道放置海绵栓,埋栓后第9天先采集血液,再将150 IU FSH按照不同注射方法对A、B、C组进行处理,第11天采集血液后,撤栓注射相同剂量的孕马血清促性腺激素(PMSG)和氯前列烯醇(PG)进行同期发情处理,在发情0 h和发情12 h采集血液,采用酶联免疫吸附测定法(ELISA)测定E2、FSH、LH和P4的浓度。[结果]FSH的浓度在FSH处理前、撤栓前、发情0 h和发情12 h时,各试验组之间血清中差异不显著(P>0.05);E2的浓度在撤栓前B、C组显著高于A、D组(P<0.05),在发情0 h时,B、C组极显著高于A组(P<0.01)、D组(P<0.01),D组极显著高于A组(P<0.01)。LH的浓度在撤栓前D组显著高于A、B、C组(P<0.05),在发情0 h,B组显著高于A、C、D组(P<0.05);P4的浓度在FSH处理前、撤栓前、发情0 h和发情12 h时,各试验组之间差异不显著(P>0.05)。[结论]外源生殖激素FSH的注射会对血液中E2和LH的浓度造成影响,且发情0 h时血液中E2的浓度与发情率呈正相关。单次FSH注射剂量150 IU可能会抑制LH和E2的分泌。75 IU+75 IU平均注射法对发情率有较好的作用;100 IU+50 IU递减注射法,在发情0 h可以明显提高血液中E2和LH的浓度。

[Objective]In order to study the effects of different FSH treatment methods on the reproductive traits of Duolang sheep.[Method]A total of 172 ewes were randomly divided into three experimental groups(A,B and C)and one control group(D).Under the same feeding conditions,a sponge plug was placed in the vagina,blood was collected on the 9th day after the plug was embedded,and then 150 IU FSH was administered to groups A,B,and C according to different injection methods.After blood was collected on the 11th day,the plug was withdrawn and injected.The same dose of pregnant horse serum gonadotropin(PMSG)and cloprostenol(PG)were treated in estrus at the same time,blood was collected at 0 h and 12 h of estrus,and E2,FSH,LH and P4 were measured by enzyme-linked immuno sorbent assay(ELISA).[Result]There was no significant difference in serum FSH concentration among the experimental groups before FSH treatment,at the time of thrombectomy,at 0 h and 12 h in estrus(P>0.05);the concentration of E2 in groups B and C were higher than that in groups A and D(P<0.05),and at 0 h of estrus,groups B and C were significantly higher than those in group A(P<0.01),and groups B and C were significantly higher than those in group D(P<0.05),group D was significantly higher than that of group A(P<0.05).The concentration of LH in group D was significantly higher than that in groups A,B,and C at the time of thrombectomy(P<0.05),and at 0 h of estrus,group B was significantly higher than that in groups A,C,and D(P<0.05);the concentration of P4 in FSH before treatment,when the thrombus was withdrawn,at 0 h and 12 h in estrus,there was no significant difference between the experimental groups(P>0.05).[Conclusion]The injection of exogenous reproductive hormone FSH will affect the concentrations of E2 and LH in blood,and the concentration of E2 in blood at 0 h of estrus was positively correlated with the estrus rate.A single FSH injection dose of 150 IU may inhibit the secretion of LH and E2.The 75 IU+75 IU injection method has a better effect on the estrus rate;the 100 IU+50 IU injection method can significantly increase the concentration of E2 and LH in the blood at 0 h of estrus.