冬荪绿霉病病原鉴定及药剂筛选

Pathogen Identification of Mold Disease of Phallus dongsun and Fungicides Selection

以采自贵州省大方县、织金县种植基地的冬荪(Phallus dongsun)绿霉病样品为试材,经病原分离和致病性测定,结合病原真菌形态特征并基于ITS、tef1、rpb2这3个基因片段进行系统发育分析,并测定该病原菌对4种杀菌剂及其2种混配药剂的敏感性,以期揭示病原种类并寻找有效药剂,为病害的综合防治研究提供借鉴。结果表明,病原真菌为绒毛木霉(Trichoderma tomentosum);4种原药中苯醚甲环唑对该菌株抑制最强,EC_(50)为1.93 mg/L,其次为吡唑醚菌酯和咪鲜胺锰盐,EC_(50)分别为4.02和4.96 mg/L;混配组合中,咪鲜胺锰盐和苯醚甲环唑1∶2时,EC_(50)为2.18 mg/L,共毒系数CTC值为150.88,具有显著的协同增效作用。

To identify the pathogen,Phallus dongsun samples with green mold were collected from Dafang County and Zhijin County,Guizhou Province.The pathogen was isolated and then identified based on pathogenicity test,morphological characteristics and multi-locus phylogenetic analysis(ITS,tef1 and rpb2).The sensitives of the pathogen to 4 fungicides and 2 mixing were evaluated.This study provides references for further integrated control of the disease.The results showed that pathogen was identified as Trichoderma tomentosum.The results of the sensitivities evaluation showed that all fungicides had inhibition effect on the pathogen,of which,difenoconazole had the best inhibition effect,EC_(50)=1.93 mg/L.The EC_(50)of pyraclostrobin and prochloraz-manganese were 4.02 and 4.96 mg/L,respectively.The mixing of prochloraz-manganese and difenoconazole(1∶2)had the best inhibition effect,EC_(50)=2.18 mg/L,CTC=150.88.