华南三省(自治区)部分西番莲种植区花叶病病原检测及EAPV多聚蛋白基因序列分析

Viral pathogens detection of Passiflora edulis mosaic disease and sequence analysis of polyprotein gene of East Asian passiflora virus in some areas of South China

西番莲(Passiflora edulis Sims.)是热带亚热带地区重要经济作物,我国福建、广东、广西、海南等地广泛种植[1]。调查发现,我国南方多地西番莲花叶病为害严重,症状表现为叶片花叶、黄化、斑驳、畸形,甚至整株褪绿,严重影响西番莲的产量和品质。

Passiflora edulis mosaic disease is a serious constraint to the production of Passiflora edulis. in China. During 2016-2019, 170 samples of P. edulis with typical mosaic symptoms were collected from 7 planting areas in Fujian, Guangxi and Guangdong. Cucumber mosaic virus(CMV), Potyvirus and Tobamovirus were detected using RT-PCR with degenerate primers and/or specific primers. The results showed that 90 out of 170 samples were detected positive. The detection rate of CMV was 51.18% while the detection rate of Potyvirus was 10.00 %, however, Tobamovirus was not found in this research. CMV and Potyvirus showed severe mixed infection which account for 15.56% of total infection rate. Further identification of viruses in the genus Potyvirus was performed and the amplified fragment shared 98.00%-100.00% identity with East Asian passiflora virus aviliable in the GenBank. Additionally, 5 isolates with polyprotein gene sequences were obtained and phylogenetic analysis of these 5 isolates indicated a high genetic relationship to the AO stain reported from Japan and China(Taiwan). These results will be helpful for the detection and effective control of Passiflora edulis mosaic disease.