人参二醇皂苷通过调节上皮间质转化抑制喉癌细胞的增殖和迁移

Panaxadiol saponins inhibits the proliferation and migration of laryngeal carcinoma cells by regulating epithelial-mesenchymal transition

目的 探讨人参二醇皂苷(PDS)对喉癌Hep2细胞增殖、迁移和上皮间质转化(EMT)的影响,并阐明其作用机制。方法 人喉癌Hep2细胞分为对照组和不同浓度的PDS(0,19,38,75,150,300 mg·L^(-1))及地塞米松(100 nmol·L^(-1))处理组,MTT实验检测细胞生长活力,HE染色观察细胞形态学改变,细胞划痕实验检测细胞迁移能力,实时定量PCR(RT-qPCR)法和Western blotting法检测β-catenin、E-cadherin、N-cadherin和vimentin的mRNA和蛋白表达水平。结果 与对照组相比,PDS以时间及剂量依赖性抑制Hep2细胞生长。与对照组相比,用150 mg·L^(-1)PDS处理喉癌Hep2细胞48 h后,可与地塞米松类似的下调细胞划痕愈合率,细胞形态向上皮型转化,上皮细胞标志物E-cadherin上调(P<0.05),间充质细胞标志物N-cadherin和vimentin下调(P<0.05),Wnt/β-catenin信号通路重要蛋白β-catenin的mRNA和蛋白表达水平明显降低(P<0.05)。结论 PDS可通过抑制Wnt/β-catenin途径,抑制Hep2细胞增殖、迁移和EMT。

Objective To investigate the effects of panaxadiol saponins(PDS) on proliferation, migration and epithelial-mesenchymal transformation(EMT) of laryngeal carcinoma Hep2 cells, and to elucidate its mechanism.Methods Human laryngeal carcinoma Hep2 cells were divided into control group and different concentrations of PDS(0,19,38,75,150,300 mg · L^(-1)) and dexamethasone(100 nmol · L^(-1)) treatment groups.MTT assay was used to detect the cell viability, HE staining was used to observe the cell morphological changes, cell scratch assay was used to detect the cell migration ability, real-time quantitative PCR(RT qPCR) and Western blotting method were used to detect mRNA and protein expression levels of β-catenin, E-cadherin, N-cadherin and vimentin.Results Compared with the control group, PDS inhibited Hep2 cell growth in a time and dose dependent manner.Compared with the control group, the laryngeal cancer Hep2 cells treated with 150 mg · L^(-1)PDS for 48 hours could reduce the rate of scratch healing similar to dexamethasone, the cell morphology transformed to epithelial type, the epithelial cell marker E-cadherin was up regulated(P<0.05),the mesenchymal cell markers N-cadherin and vimentin were down regulated(P<0.05),The mRNA and protein expression levels of β-catenin, an important protein of Wnt/β-Catenin signal pathway, decreased significantly(P<0.05).Conclusion PDS can inhibits Hep2 cell proliferation, migration and EMT by inhibiting Wnt/β-Catenin pathway.