低温蛋白酶研究进展与应用

Progress and Application of Cold-active Protease

低温蛋白酶是一类能够在低温下将大分子蛋白质水解为小分子多肽或者氨基酸的水解酶,多由低温环境中的微生物产生。低温蛋白酶在较低温度环境下具有较高的催化活性,在反应过程中仅较少的量就可以达到理想效果,并且可以避免不必要的化学转化,减少挥发性物质的损失。低温蛋白酶在洗涤、食品加工以及医疗等应用领域已经显示出独特的优势和商业价值。综述不同种类及来源的微生物低温蛋白酶,总结其催化性质、结构特征以及目前的应用情况和未来发展前景,以期进一步推动新型低温蛋白酶的开发和应用。

Cold-active protease is a kind of hydrolytic enzyme that can hydrolyze large proteins into small peptides or amino acids at low temperatures.It is generally produced by microorganisms living at low temperatures and has high catalytic activity at lower ambient temperatures.Therefore,it can avoid waste of resources caused by the rise in temperature in the application process and the special requirements for equipment,and shows unique advantages and commercial value in the application fields of washing,food processing and medical treatment.In this paper,different kinds and sources of microbial cryopinases are reviewed,and their catalytic properties,structural characteristics,current application and future development prospects are analyzed and summarized based on existing research results,aiming to promote the development and application of new cryopinases.