改良椎板切除法构建脊髓损伤模型大鼠

Improved laminectomy for constructing a rat model of spinal cord injury

背景:目前脊髓损伤已成为世界性难题,实验动物造模是探索疾病的第一步,但现缺乏较为有效的实验动物模型。目的:建立一种可复制、可调控、创伤小、死亡率低、出血量少、适用范围广、术中时间短的脊髓损伤大鼠模型。方法:将选用的体质量相似的40只SD大鼠随机分为常规组和改进组,每组20只,常规组采用Allen法构建脊髓损伤模型,改良组在原有模型的基础上,运用牙科磨钻代替器械咬除,比较两组大鼠手术时间、术中出血量、死亡率及击打后1,3,6,9,12,15 d的BBB运动功能学评分。结果与结论:改良继发性脊髓损伤模型建立方法比常规建模方法出血量少、死亡率低、造模时间短、BBB评分结果更集中,由此可证明改良后的击打方法更适用于继发性脊髓损伤模型的建立。

BACKGROUND:Spinal cord injury has become a worldwide problem.Experimental animal modeling is the first step to explore the disease;however,there is a lack of experimental animal models that are more effective.OBJECTIVE:To establish a rat model of spinal cord injury that can be replicated,regulated,has little trauma,low mortality,low blood loss,wide application and short intraoperative time.METHODS:Forty Sprague-Dawley rats with similar body mass were selected and randomly divided into conventional model group and improved model group(n=20 per group),where the model of spinal cord injury was made using Allen’s method and improved method,respectively.In the improved model group,a dental drill was used for biting.Operation time,intraoperative blood loss,mortality,and Basso-Beattie-Bresnahan locomotor rating scale scores at 1,3,6,9,12,and 15 days after beating were compared between the two groups.RESULTS AND CONCLUSION:The improved method for constructing the animal model of secondary spinal cord injury has the advantages of shorter modeling time,less intraoperative blood loss,lower mortablity,and more centralized Basso-Beattie-Bresnahan score data as compared with the conventional method,which proves that the improved method is more suitable for establishing the model of secondary spinal cord injury.